花吊丝竹居群遗传多样性的ISSR分析  

Genetic Diversity Analysis of Dendrocalamus minor var. amoenus Populations Based on ISSR Markers

作　　者：瞿印权 杨德明[1] 徐雯[1] 杜溶讫 何天友[2] 施成坤[3] 荣俊冬[1] 陈礼光[1] 郑郁善[1] QU Yin-quan;YANG De-ming;XU Wen;Du Rong-qi;HE Tian-you;SHI Cheng-kun;RONG Jun-dong;Chen Li-guang;ZHENG Yu-shan(College of Forestry,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China;College of Art&Landscape Architecture,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China;Chishan State-owned Forest Farm,Dongshan 363400,Fujian,China)

机构地区：[1]福建农林大学林学院,福建福州350002 [2]福建农林大学艺术学院园林学院,福建福州350002 [3]福建省东山赤山国有防护林场,福建东山363400

出　　处：《林业科学研究》2018年第6期33-38,共6页Forest Research

基　　金：福建省区域发展项目(2015N3015);福建省科技重大专项(2013NZ0001);福建省科技重大项目(2010N5002)

摘　　要：[目的]研究花吊丝竹居群遗传多样性和遗传结构,为种质资源有效利用和良种选育提供理论指导。[方法]利用12条ISSR引物对48份种质(共3个居群)花吊丝竹居群进行遗传多样性和遗传距离分析。[结果]共检测到124个位点,其中,多态性位点为102个,种质和居群水平上的多态位点百分比(PPB)分别为82. 26%和50. 27%,Ne’基因多样性指数(He)分别为0. 220 4和0. 206 6,Shannon’s信息指数(I)分别为0. 349 4和0. 300 5,表明花吊丝竹居群间存在中等水平的遗传变异。根据Nei’s遗传多样性计算出不同居群间分化水平(Gst)=0. 163 3,表明16. 33%的遗传变异存在于居群间,居群内的遗传变异为83. 67%。居群间的基因流Nm为2. 562 1,表明花吊丝竹居群间存在较大基因流,很大程度减少居群间遗传差异。基于遗传距离的UPGMA聚类结果表明,48份种质可分为3组,3个居群可分为2组,居群间地理距离与亲缘关系无显著相关性。[结论]虽然花吊丝竹主要靠营养生殖来繁衍后代,其居群遗传多样性较丰富,且居群内遗传多样性大于居群间。此外,福建居群遗传多样性明显高于广西和广东地区居群。[Objective]To reveal the genetic diversity and genetic structure of Dendrocalamus minor var.amoenus populations and provide useful information on effective utilization of germplasm resources and elite breeding.[Method]The genetic diversity and genetic distance of 48 D.minor var.amoenus individuals from 3 populations were analyzed by using 12 ISSR primers.[Result]A total of 124 loci were identified,of which 102 loci were polymorphism,accounting for 82.26%,Nei’s ISSR diversities(He)at the species and population level were 0.220 4 and 0.206 6,respectively,the Shannon’s information index(I)were 0.349 4 and 0.300 5,indicating that D.minor var.amoenus possessed medium level of genetic diversity.The variance within populations was the main part of the genetic variation according to Gst analysis.The level of gene flow(Nm)was calculated as 2.562 1 individuals per generation within the 3 populations,indicating that the majority of genetic variation occurred within populations.The UPGMA tree based on genetic distance(D)indicated that 48 individuals could be divided into 3 types and 3 populations could be clustered into 2 groups.The clustering in this dendrogram was not consistent with the geographical distribution,suggesting that there was no obvious correlation between genetic distance and geographic distance of populations.[Conclusion]Analysis of 3 populations based on the index of the Nei’s genetic diversity showed that the genetic diversity of D.minor var.amoenus individuals is abundant though it is an asexual plant.The genetic diversity of species within populations is higher than that among populations.Fujian’s population shows the highest level of genetic diversity and it should be a priority for protection.

关 键 词：花吊丝竹 遗传多样性 ISSR 

分 类 号：S718.46[农业科学—林学] S795.9