云南甜龙竹和黄竹种子灭菌条件和萌发特性初步研究  

Optimum Sterilization Conditions and Germination Characteristics of Dendrocalamus brandisii and D. membranaceus Seeds

作　　者：郑祥亁 陈凌娜[1] 崔永忠[1] 杨汉奇[1] ZHENG Xiang-qian;CHEN Ling-na;CUI Yong-zhong;YANG Han-qi(Research Institute of Resources Insects,Chinese Academy of Forestry,Kunming 650233,Yunnan,China)

机构地区：[1]中国林业科学研究院资源昆虫研究所,云南昆明650233

出　　处：《林业科学研究》2018年第6期76-82,共7页Forest Research

基　　金：中央级公益性科研院所基本科研业务费专项(CAFYBB2017ZX001-8;CAFYBB2017QA014);国家科技部(2018ZX08024001-002-002);西藏科技厅(XZ201801-GA-11);云南省科学技术厅(2014HB041)

摘　　要：目的]筛选云南甜龙竹和黄竹种子最佳表面灭菌条件,研究其萌发特性。[方法]筛选NaClO(2%、3%、4%)与HgCl_2(0.01%、0.1%)最佳消毒浓度组合;采用流水冲洗时间(6、12、18 h)、2%NaClO浸泡时间(5、10、15 min)和0.1%Hg Cl_2浸泡时间(5、10、15 min),设计正交试验实验,探讨2种竹种最佳表面灭菌处理的时间组合,并对竹种分别在滤纸、MS培养基以及经3 mg·L^(-1)GA_3浸泡后在MS培养基上的发芽率进行了探讨。[结果]NaClO、Hg Cl_2最佳消毒浓度组合为2%NaClO+0.1%HgCl_2;2种竹种最佳表面灭菌时间组合均为:流水冲洗6 h、2%Na Cl O浸泡10min、0.1%HgCl_2浸泡15 min,经此处理后云南甜龙竹和黄竹的发芽率分别为84.4%、72.2%,污染率分别为18.7%、29.6%。不同处理种子萌发率差异t检验结果显示:2种竹子种子是否经过3 mg·L^(-1)GA_3浸泡,对其在MS培养基上的发芽率无显著影响,但种子在MS培养基上的发芽率显著高于滤纸上的发芽率。经相同处理黄竹的发芽率均低于云南甜龙竹。[结论]云南甜龙竹、黄竹最佳表面灭菌组合均为:流水冲洗6 h、2%NaClO浸泡10 min、0.1%HgCl_2浸泡15 min;3 mg·L^(-1)GA_3浸泡处理对云南甜龙竹和黄竹种子在MS培养基上的发芽率无显著影响,但MS培养基上种子的发芽率显著高于其在滤纸上的发芽率。[Objective]To study the best surface sterilization conditions and germination characteristics of Dendrocalamus brandisii(Munro)Kurz and D.membranaceus Munro seed.[Method]NaClO(1%,2%,3%)and HgCl 2(0.01%,0.1%)were used to sterilize the seeds of the two species so as to find out the most suitable combination of disinfectant conditions.Three factors,namely the water flushing time(6,12,18 h),2%NaClO soaking time(5,10,15 min)and 0.1%HgCl 2 soaking time(5,10,15 min),were used to design the experiments by the L 9(3 4)orthogonal table for selecting the best treatment combination.The seed germination rates using filter medium,MS medium and MS medium after soaked in 3 mg·L^-1 GA 3 were investigated respectively.[Result]The optimum combination of disinfectant conditions was 2%NaClO+0.1%HgCl 2.The best sterilization treatment combination for these two bamboos seeds were:6 h water flushing time,10 min 2%NaClO soaking time and 15 min 0.1%HgCl 2 soaking time.After treated,the germination rates of D.brandisii and D.membranaceus seeds were 84.4%and 72.2%respectively,and the pollution rate of the seeds were 18.7%and 29.6%respectively.The results of t test showed that no significant difference was found in germination rate of D.brandisii seeds between on MS medium and on MS medium after seeds soaked in 3 mg·L^-1 GA 3,the germination rate reached 85.6%and 84.4%,respectively.However,the seed germination rate on two MS mediums were significantly higher than that on filter paper(71.1%).The seed germination rate of D.membranaceus was lower than that of D.brandisii,and also displayed similar germination rule for three mediums.[Conclusion]The best sterilization treatment combination for D.brandisii and D.membranaceus seeds are:6 h water flushing time,10 min 2%NaClO soaking time and 15 min 0.1%HgCl 2 soaking time.There is no significant difference in germination rate of D.brandisii and D.membranaceus seeds between on MS medium and on MS medium after seeds soaked in 3 mg·L^-1 GA 3.However,the seed germination rate on two MS mediums were s

关 键 词：云南甜龙竹 黄竹 种子萌发 表面灭菌 组织培养 

分 类 号：S722.1[农业科学—林木遗传育种]