罗丹明B荧光猝灭法测定水中碘  

Determination of iodide ions with rhodamine B based on the fluorescence quenching

作　　者：乔玉春 龙大成 孙宗招 郑滢婷 陈丽君 王桦 Qiao Yu-chun;Long Da-cheng;Sun Zong-zhao;Zheng Ying-ting;Chen Li-jun;Wang Hua(The Key Laboratory of Life-Organic Analysis, Qufu Normal University, Qufu 273165, China;Jining Functional Materials and Monitoring Devices E&T Center, Jining 272000, China)

机构地区：[1]曲阜师范大学化学与化工学院山东省生命有机分析重点实验室,山东曲阜273165 [2]济宁功能材料与监测器件工程技术中心山东三生新材料科技有限公司,山东济宁272000

出　　处：《化学传感器》2018年第1期39-43,共5页Chemical Sensors

基　　金：山东省自然科学基金计划(ZR2017PB009);国家科技部"星火计划"(2015GA105005)资助

摘　　要：碘是人体不可或缺的一种微量元素,目前众多的含碘食品进入人们生活中。碘过量或缺乏都会对人体产生不良的影响。罗丹明B作为最常用的荧光染料被广泛用于分析化学和生物医药领域。研究表明,在酸性条件下碘离子与碘酸根反应生成碘单质,碘单质与罗丹明B反应导致其荧光猝灭,由此建立了一种简便的碘离子荧光分析方法,并发现罗丹明B荧光猝灭值与5.0～60μg/L碘离子的浓度范围内呈良好的线性关系。该碘离子检测方法具有灵敏度高、检出限低、选择性好、操作方便等优点。Iodine is a kind of indispensable trace element in human body.Nowadays,iodine-containing food en-tered into the people's life,and it can also cause many bad effects when iodine is excessive or lacking.Fluorescent dye Rhodamine B commonly used in analytical chemistry and biological medicine fields.The reaction between io-dine ions and iodate ions could be occurred under acid condition,and then generate I2reacting with rhodamine B which resulted in the fluorescence quenching.Therefore,a fluorescence quenching-based determination method was developed for probing iodide ions.It was found that the fluorescence quenching efficiency exhibited a linear re-lation with the iodine ions concentrations ranging from5.0to60^-g/L.The developed detection method showed many excellent characteristics with high sensitivity,low detection limit,good selectivity and rapid operation.

关 键 词：碘离子 罗丹明B 荧光猝灭法 

分 类 号：X832[环境科学与工程—环境工程] O657.3[理学—分析化学]