3D打印技术构建不同比例聚己内酯-磷酸三钙支架及其体外诱导成骨性能  

Construction of polycaprolactone-tricalcium phosphate with different mixture ratios using three-dimensional printing technology and its osteoinductivity in vitro

作　　者：苑博 王智巍[1] 唐一钒 周盛源[1] 陈雄生[1] 贾连顺[1] Yuan Bo;Wang Zhiwei;Tang Yifan;Zhou Shengyuan;Chen Xiongsheng;Jia Lianshun(Spine Center,Department of Orthopedics, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai 200003, China)

机构地区：[1]解放军第二军医大学上海长征医院骨科医院脊柱外科,上海市200003

出　　处：《中国组织工程研究》2019年第6期821-826,共6页Chinese Journal of Tissue Engineering Research

基　　金：上海市科委基础研究重点项目(15JC1491003);项目负责人:陈雄生~~

摘　　要：背景:骨组织工程支架的复合材料选择及混合比例对于支架成骨诱导作用的影响巨大。目的:借助3D打印技术构建不同混合比例的聚己内酯-磷酸三钙复合支架,探究其物理特征和成骨诱导能力。方法:将聚己内酯(polycaprolactone,PCL)和磷酸三钙(tricalcium phosphate,TCP)分别按照10∶0、9∶1、8∶2、7∶3的质量比混合,借助3D打印技术构建单纯聚己内酯支架和聚己内酯-磷酸三钙复合支架,分别命名为单纯PCL支架、PCL-10%TCP支架、PCL-20%TCP支架、PCL-30%TCP支架,利用扫描电子显微镜和疲劳试验机表征支架的微观结构和力学性能。将兔骨髓间充质干细胞分别接种于单纯PCL支架、PCL-10%TCP支架、PCL-20%TCP支架上,接种后第1,4,7天检测细胞增殖,第1,7,14天检测细胞内碱性磷酸酶活性,第7,14,21天检测支架上清液内的钙含量。结果与结论:(1)PCL-30%TCP支架无法通过该方式构建支架;(2)与单纯PCL支架相比,PCL-10%TCP支架、PCL-20%TCP支架的抗压强度和弹性模量更强(P<0.05),孔隙率无明显改变;(3)单纯PCL支架、PCL-10%TCP支架、PCL-20%TCP支架均可观察到预先设计的孔隙结构,支架内部孔隙相互连通;(4)单纯PCL支架、PCL-10%TCP支架、PCL-20%TCP支架均可促进骨髓间充质干细胞增殖,PCL-20%TCP支架组接种第4,7天的细胞增殖效果优于其余两组支架(P <0.05);(5)PCL-20%TCP支架组接种第7,14天的细胞内碱性磷酸酶活性高于其余两组支架(P <0.05);(6)接种第14天,PCL-20%TCP支架组钙含量高于其余两组支架(P<0.05);(7)结果表明,相比于其他支架,PCL-20%TCP支架具有更好的力学性能,更能促进骨髓间充质干细胞的增殖和成骨分化。BACKGROUND:The choice and mixture ratio of composite materials for bone tissue engineering scaffolds have a great influence on the osteoinductivity of scaffolds.OBJECTIVE:To construct the polycaprolactone-tricalcium phosphate composite scaffolds with different mixture ratios using3D printing technology,and to test their physiochemical characteristics and osteoinductivity.METHODS:Polycaprolactone and tricalcium phosphate were mixed in different ratios of10:0,9:1,8:2,and7:3,and the polycaprolactone and polycaprolactone-tricalcium phosphate scaffolds were constructed by the3D printing technology.The scaffolds were named as PCL scaffold,PCL-10%TCP scaffold,PCL-20%TCP scaffold,PCL-30%TCP scaffold.The microstructure and mechanical properties of the scaffolds were characterized by scanning electron microscopy and fatigue testing machine.The proliferation and alkaline phosphatase activity of rabbit bone marrow mesenchymal stem cells seeded on different scaffolds were detected at1,4,7days of culture and at1,7and14days of culture,respectively,by in vitro experiments.The content of calcium in the supernatant of the scaffolds was detected at7,14,and21days of culture RESULTS AND CONCLUSION:The PCL-30%TCP scaffold could not be constructed by this method.Compared with the PCL scaffold,the compressive strength and elastic modulus of the PCL-10%TCP and PCL-20%TCP composite scaffolds were stronger(P<0.05),and the porosity did not change significantly.Pre-designed pore structure could be observed in the PCL,PCL-10%TCP and PCL-20%TCP scaffolds,and the internal pores of the scaffolds were interconnected.Bone marrow mesenchymal stem cells could proliferate on all the three scaffolds.The proliferation rate of the cells on the PCL-20%TCP scaffold was better than that of the other two scaffolds at4and7days of culture(P<0.05).The alkaline phosphatase activity(7and14days of culture)and calcium content(14days of culture)of bone marrow mesenchymal stem cells on the PCL-20%TCP scaffold were significantly higher than those of the PCL and PCL-1

关 键 词：3D打印 聚己内酯 磷酸三钙 复合支架 力学性能 成骨分化 细胞内碱性磷酸酶活性 生物相容性材料 间质干细胞 细胞增殖 碱性磷酸酶 组织工程 

分 类 号：R459.9[医药卫生—治疗学] R318.08[医药卫生—临床医学]