改良型富血小板纤维蛋白复合β-磷酸三钙诱导的新生骨整合  

Osseointegration induced by beta-tricalcium phosphate loaded with advanced platelet-rich fibrin

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作  者:刘丹 闵昌琴 芦帅 陈跃 孙勇 Liu Dan;Min Changqin;Lu Shuai;Chen Yue;Sun Yong(Hospital of Stomatology, Southwest Medical University, Luzhou 646000, Sichuan Province, China;Sichuan Hospital of Stomatology, Chengdu 610031, Sichuan Province, China;Chengdu Wenjiang District People’s Hospital,Chengdu 611130, Sichuan Province, China;Institutional Hospital of Chengdu Military Region, Chengdu 610031, Sichuan Province, China;Department of Nuclear Medicine, the Affiliated Hospital of Southwest Medical University, Luzhou 646000, Sichuan Province, China)

机构地区:[1]西南医科大学附属口腔医院,四川省泸州市646000 [2]四川口腔医院,四川省成都市610031 [3]成都市温江区人民医院,四川省成都市611130 [4]成都市军区机关医院,四川省成都市610031 [5]西南医科大学附属医院核医学科,四川省泸州市646000

出  处:《中国组织工程研究》2019年第6期888-893,共6页Chinese Journal of Tissue Engineering Research

基  金:军事口腔国家重点实验室开放课题(2016KB01);项目负责人:孙勇~~

摘  要:背景:前期兔临界性骨缺损修复实验显示,β-磷酸三钙与改良型富血小板纤维蛋白复合材料可诱导新生骨形成,促进骨组织的修复,效果优于单独使用β-磷酸三钙或改良型富血小板纤维蛋白。目的:进一步探索β-磷酸三钙与改良型富血小板纤维蛋白复合材料引导的新生骨与种植体骨整合性能。方法:取18只日本大耳兔(成都达硕生物科技有限公司提供),建立双侧股骨髁部骨缺损模型(直径6 mm、深度10mm),随机分3组:β-磷酸三钙组将β-磷酸三钙材料填入骨缺损处,改良型富血小板纤维蛋白组将改良型富血小板纤维蛋白填入骨缺损处,复合组将质量比为1∶1的β-磷酸三钙与改良型富血小板纤维蛋白混匀,填入骨缺损处;材料植入后3个月,植入DIO种植体,种植体植入后第4,12周获取带种植体的新生骨标本,进行Micro-CT及种植体推出实验,检测骨结合界面质量与推出最大荷载值。结果与结论:(1)Micro-CT检测显示,随着时间的延长,各组新生骨量逐渐增加,并且复合组植入后第4,12周的新生骨量多于β-磷酸三钙组、改良型富血小板纤维蛋白组(P <0.05);(2)种植体推出实验显示,随着时间的延长,各组最大推出力增加,并且复合组植入后第4,12周的最大推出力高于β-磷酸三钙组、改良型富血小板纤维蛋白组(P <0.05);(3)结果表明相对于单独应用,β-磷酸三钙与改良型富血小板纤维蛋白复合材料可优化新骨与种植体的结合。BACKGROUND:Preliminary studies have shown thatβ-tricalcium phosphate loaded with advanced platelet-rich fibrin can induce bone formation and promote bone repair,which is better thanβ-tricalcium phosphate or advanced platelet-rich fibrin alone.OBJECTIVE:To further explore the osseointegration properties of newly formed bone induced byβ-tricalcium phosphate loaded with advanced platelet-rich fibrin.METHODS:Eighteen Japanese rabbits provided by Chengdu Dashuo Biotechnology Co.,Ltd.in China were used for establishing the bilateral femoral condyle defect models(6mm in diameter and10mm in depth).There were three groups implanted with advanced platelet-rich fibrin,β-tricalcium phosphate orβ-tricalcium phosphate loaded with advanced platelet-rich fibrin(mass ratio1:1)composite,respectively.At3months after implantation,the DIO implant was implanted.The newly bone samples with the implants were then obtained to perform micro-CT and push-out test of implants at4and12weeks.The osteogenic properties and maximum load of the implant-bone interface were detected.RESULTS AND CONCLUSION:Micro-CT findings revealed that the amount of newly formed bone in each group was increased with time,which was significantly higher in the combined group than the other two groups at4and12weeks after implantation(P<0.05).Results from the push-out test of implants showed that as time went on,the maximum push strength of each group was increased,which was significantly higher in the combined group than in the other two groups at4and12weeks after implantation(P<0.05).In summary,the combined strength of the implant-bone interface ofβ-tricalcium phosphate loaded with advanced platelet-rich fibrin is superior to that of advanced platelet-rich fibrin orβ-tricalcium phosphate.

关 键 词:改良型富血小板纤维蛋白 Β-磷酸三钙 种植体骨结合 推出最大荷载 新生骨整合 纤维蛋白 磷酸钙类 牙种植体 骨结合 组织工程 

分 类 号:R459.9[医药卫生—治疗学] R318.19[医药卫生—临床医学]

 

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