Triton-x100与丹参酚酸B制备脱细胞血管支架及其生物力学性能  

Preparation of acellular vascular scaffold using Triton-x100 and salvianolic acid B and its biomechanical performance

作　　者：赵亮[1,2] 李霞飞 周坤 闫欢欢 张其清 Zhao Liang;Li Xiafei;Zhou Kun;Yan Huanhuan;Zhang Qiqing(College of Engineering, Peking University,Beijing 100871, China;School of Life Sciences and Technology, Life Science and Health Research Institute,Xinxiang Medical University, Xinxiang 453003, Henan Province, China;School of Biomedical Engineering,Xinxiang Medical University, Xinxiang 453003, Henan Province, China)

机构地区：[1]北京大学工学院,北京市100871 [2]新乡医学院生命科学技术学院生命科学与健康研究院,河南省新乡市453003 [3]新乡医学院生物医学工程学院,河南省新乡市453003

出　　处：《中国组织工程研究》2019年第6期951-956,共6页Chinese Journal of Tissue Engineering Research

基　　金：河南省高等学校重点科研项目计划(17A180034);项目负责人:赵亮;第二届大学生生命科学联赛立项课题(SKYLS017);项目负责人:赵亮~~

摘　　要：背景:脱细胞血管支架具有优良性能,但是在其制备过程中其生物力学性能出现不同程度的降低。目的:观察在组织工程制备脱细胞血管支架中丹参酚酸B的应用前景。方法:经Triton-x100单独处理,Triton-x100-丹参酚酸B联合处理制备脱细胞血管支架,分别设为Tx组和Tx-sal组,对其进行形态学观察、组织厚度测量、孔隙率检测及生物力学特性检测,比较在应用丹参酚酸B后处理之后脱细胞血管支架在形态学等各种指标上的变化。结果与结论:Tx组和Tx-sal组的血管管壁厚度及血管孔隙率差异无显著性意义(P> 0.05),形态学无明显变化。Tx-sal组的脱细胞血管支架可以较完好地保留血管壁的胶原纤维和弹性纤维,抗拉强度、最大变形量、断裂伸长比、爆裂压力及缝合强度均优于Tx组(P <0.05)。结果证实,Triton-x100联合丹参酚酸B处理能够成功制备脱细胞效果良好、生物力学性能优良的脱细胞血管支架。BACKGROUND:The acellular vascular scaffold has excellent properties,but its biomechanical properties have been reduced to varying degrees during preparation.OBJECTIVE:To explore the application prospect of salvianolic acid B in the tissue-engineered preparation of acellular vascular scaffold.METHODS:The rat thoracic aorta was decellularized using Triton-x100alone(Tx group)or combined with salvianolic acid B(Tx-sal group)to prepare acellular vascular scaffolds.Morphology of the two groups of acellular vascular scaffolds was observed.Measurements of tissue thickness,porosity and biomechanical properties were conducted,and changes in the morphology of acellular vascular scaffolds were compared between two groups.RESULTS AND CONCLUSION:The use of salvianolic acid B treatment had no effect on the wall thickness and porosity of acellular vascular scaffolds,and there was no significant difference between the Tx group and Tx-sal group(P>0.05).There was also no significant change in the scaffold morphology.Compared with the Tx group,the acellular vascular scaffold in the Tx-sal group was better to retain collagen fibers and elastic fibers of the vessel wall.The biomechanical indicators in the Tx-sal gorup such as tensile strength,maximum deformation,elongation at break,burst pressure,and suture strength were all superior to those in the Tx group(P<0.05).Therefore,Triton-x100combined with salvianolic acid B treatment can produce acellular vascular materials with good decellularization and excellent biomechanical properties.

关 键 词：丹参酚酸B 脱细胞血管支架 血管移植材料 孔隙率 抗拉强度 主动脉 细胞-材料复合物 缝合强度 组织构建 支架 生物力学 组织工程 

分 类 号：R456[医药卫生—治疗学] R318[医药卫生—临床医学]