单向灌流法研究姜黄素羟丙基-β-环糊精包合物的大鼠在体肠吸收  

Intestinal absorption characteristics of curcumin hydroxypropyl-beta-cyclodextrin in rats: an analysis using one-way perfusion method

作　　者：罗见春 郭绮 张敏[1] 钟萌 万胜利 张景勍[1] Luo Jianchun;Guo Qi;Zhang Min;Zhong Meng;Wan Shengli;Zhang Jingqing(Medicine Engineering Research Center, Chongqing Medical University, Chongqing 400016, China;Center for Certification and Evaluation, Chongqing Food and Drug Administration, Chongqing 401120, China;Department of Pharmacy, the Affiliated Hospital of Southwest Medical University, Luzhou 646009, Sichuan Province, China)

机构地区：[1]重庆医科大学药物高校工程研究中心,重庆市400016 [2]重庆市药品技术审评认证中心,重庆市401120 [3]西南医科大学附属医院药剂科,四川省泸州市646009

出　　处：《中国组织工程研究》2019年第6期957-962,共6页Chinese Journal of Tissue Engineering Research

基　　金：重庆市科委项目(cstc2017shmsA 0174);项目负责人:张景勍~~

摘　　要：背景:姜黄素具有良好的抗癌、抗炎等药理作用,但水溶性极低,影响了其药理活性的发挥。目的:观察姜黄素羟丙基-β-环糊精包合物及姜黄素在体肠吸收情况。方法:采用研磨法制备姜黄素羟丙基-β-环糊精包合物;检测姜黄素羟丙基-β-环糊精包合物与姜黄素的溶解度。取12只雄性SD大鼠,建立在体肠段单向灌流模型(每只大鼠选取十二指肠、空肠、回肠及结肠4个肠段),随机分2组进行药物灌流,实验组灌流姜黄素羟丙基-β-环糊精包合物水溶液,对照组灌流姜黄素混悬液,收集各肠段的灌流药液,采用紫外可见分光光度法测定姜黄素羟丙基-β-环糊精包合物及姜黄素在大鼠体内各肠段的吸收速率常速(K_a)和有效渗透率(P_(app))。结果与结论:姜黄素羟丙基-β-环糊精包合物在水中的溶解度较姜黄素提高了33.68倍;姜黄素羟丙基-β-环糊精包合物的吸收速率常数K_a结果为:十二指肠>回肠>空肠>结肠,有效渗透率P_(app)结果为:结肠>十二指肠>回肠>空肠,并且姜黄素羟丙基-β-环糊精包合物在大鼠各肠段的吸收较姜黄素明显提高(P <0.05);结果表明,姜黄素羟丙基-β-环糊精包合物能够明显改善姜黄素在大鼠肠内的吸收情况。BACKGROUND:Curcumin has good anticancer and anti-inflammatory effects,but which is limited for the low solubility.OBJECTIVE:To investigate the intestinal absorption characteristics of curcumin hydroxypropyl-β-cyclodextrin and curcumin in rats.METHODS:The curcumin hydroxypropyl-β-cyclodextrin was prepared by grinding method,and the solubility of curcumin hydroxypropyl-β-cyclodextrin and curcumin was detected.Twelve male Sprague-Dawley rats were used to establish a one-way perfusion model of intestinal segment(duodenum,jejunum,ileum and colon),and randomly divided into two groups.The experimental group was given curcumin hydroxypropyl-β-cyclodextrin,and the control group was perfused with curcumin suspension.The absorption rate(Ka)and effective permeability(Papp)of curcumin hydroxypropyl-β-cyclodextrin and curcumin at each intestinal segment were measured by ultraviolet-visible spectrophotometry.RESULTS AND CONCLUSION:The solubility of curcumin hydroxypropyl-β-cyclodextrin in water was increased by33.68times compared with that of curcumin.The absorption rate constant(Ka)of curcumin hydroxypropyl-β-cyclodextrin was ranked as follows:duodenum>ileum>jejunum>colon,and the effective permeability(Papp)was ranked as follows:colon>duodenum>ileum>jejunum,and the absorption in the intestines was significantly higher than that of curcumin(P<0.05).In summary,curcumin hydroxypropyl-β-cyclodextrin can obviously improve the absorption of curcumin in the intestines of rats.

关 键 词：姜黄素 姜黄素羟丙基-β-环糊精 体内肠吸收 紫外可见分光光度法 环糊精包合物 吸收速率 β环糊精类 肠吸收 组织工程 

分 类 号：R459.9[医药卫生—治疗学] R318.08[医药卫生—临床医学]