红苞凤梨嵌合体组织细胞学观察与叶绿素合成特性研究  

Histocytological observation and chlorophyll syntheses characterization of chimera variety of Ananas bracteatus

作　　者：刘和平[1,2] 曹莉 杨转英[4] 张俊丽[1] 林剑波 何业华[3] LIU He-ping;CAO Li;YANG Zhuan-ying;ZHANG Jun-li;LIN Jian-bo;HE Ye-hua(Department of Food and Environmental Engineering, Yangjiang Polytechnic, Yangjiang 529566, China;College of Horticulture, South China Agricultural University, Guangzhou 510642, China;Agricultural College, Guangdong Ocean University, Zhanjiang 524088, China;Institute of Fruit Science in Maoming, Maoming 525000, China)

机构地区：[1]阳江职业技术学院食品与环境工程系,广东阳江529566 [2]茂名市水果科学研究所,广东茂名525000 [3]华南农业大学园艺学院,广东广州510642 [4]广东海洋大学农学院,广东湛江524088

出　　处：《广东农业科学》2018年第11期35-39,174,共6页Guangdong Agricultural Sciences

基　　金：广东省自然科学基金(2014A030307020);2016广东省高等职业教育专业领军人才培养对象;2016广东省“扬帆计划”博士后扶持项目

摘　　要：以红苞凤梨为研究对象,通过组织细胞学观察结合叶绿素及其合成前体物质的含量测定,阐述嵌合叶片绿、白组织在组织细胞学和叶绿素合成代谢上的差异,以探索嵌合性状形成的结构和生理基础。组织细胞学观察发现,嵌合叶片绿色部分细胞含有大量的叶绿体,而白化组织中基本没有观察到叶绿体。红苞凤梨金边、金心嵌合叶片的白化组织以及全白植株叶片的叶绿素含量都低于全绿植株叶片(对照);红苞凤梨金边嵌合体与金心嵌合体叶片白化组织的叶绿素合成前体物质含量均在UroⅢ开始下降。全白植株的叶绿素合成前体物质含量则从ALA开始就显著低于全绿植株。叶绿素合成代谢下降,叶绿素含量降低,导致出现白化现象,金边、金心等品种植株叶片则表现出嵌合特性。The histocytological features and chlorophyll biosynthesis process were studied to illustratethe structural and physiological basis of the chimera variety formation mechanism in Ananas bracteatus. Thehistocytological observation demonstrated that abundant chloroplasts existed in the cells of green parts of chimericleaves, while few chloroplast was found in the white parts of chimeric leaves. Furthermore, the chlorophyll contentsin the white tissues of chimeric leaves and the complete white leaves were significantly lower than that in the greentissues of chimeric leaves and the complete green leaves. For the white leave tissues of A. bracteatus cv. Lidenii and A.bracteatus cv. Massangeana, the decline of chlorophyll precursors contents occurred in the Uro Ⅲ biosynthesis step .The decrease of chlorophyll precursors contents began from ALA in the complete white plants. Therefore, the albinoleaves would be caused by the reduction of chlorophyll biosynthesis metabolism, which can lead to a lower chlorophyllcontent.

关 键 词：红苞凤梨 嵌合体 叶绿素合成 叶绿体 白化 

分 类 号：S682.3[农业科学—观赏园艺]