肾复康对肾纤维化模型大鼠转化生长因子-β_1、Smad2、Smad7表达的影响  

Effects of Shenfukang on the Expression of TGF-β_1,Smad2 and Smad7 in Renal Fibrosis Model Rats

作　　者：刘劲松 林哲 郑燕姣 Liu Jinsong;Lin Zhe;Zheng Yanjiao(Department of Nephrology,Affiliated Hospital of Hunan Institute of TCM Hospitals,Changsha 410006,China)

机构地区：[1]湖南省中医院研究院附属医院肾内科,长沙410006

出　　处：《世界中医药》2018年第12期3095-3098,3102,共5页World Chinese Medicine

基　　金：湖南省中医药研究院基金项目(201605);湖南省自然科学基金面上项目(2017JJ3185)

摘　　要：目的:评价肾复康对纤维化模型大鼠的疗效和对肾组织转化生长因子-β_1(TGF-β_1)、Smad2、Smad7表达的影响。方法:选取SD大鼠70只,完全随机分为假手术组、肾复康组及模型组,其中肾复康组及模型组均接受肾间质纤维模型制备,将造模第2天肾复康组接受不同剂量肾复康灌胃,对照组及假手术组接受同等体积的生理盐水灌胃,均干预21 d。干预结束收集各组24 h尿液,检测24 h尿蛋白(24 UP)、血肌酐(SCr)、血尿素氮(BUN)水平的变化,并断头取材,用苏木精-伊红(HE)染色评价各组肾组织的病理变化,免疫组化法检测各组肾组织TGF-β_1的变化,用Western blotting法检测Smad2、Smad7水平。结果:与假手术组比较,肾复康组及模型组24 UP、SCr及BUN水平明显升高,差异有统计学意义(P <0. 05);与模型组比较,肾复康组24 UP、SCr及BUN水平有所下降,差异有统计学意义(P <0. 05),且随着肾复康剂量升高趋势更明显。与假手术组比较,肾复康组及对模型组肾组织病理学评分均明显升高,其中肾复康组评分低于对照组,差异有统计学意义(P <0. 05)。随着肾复康剂量的增加病理学评分逐渐下降;假手术组肾组织仅有少量TGF-β_1、Smad2表达,模型组Smad2、TGF-β1呈高表达,随着肾复康剂量的增加Smad2、TGF-β_1水平逐渐下降。造模后Smad7水平下降,肾复康干预后Smad7水平有所上调,并随着水平的增加而上调趋势更明显。结论:肾复康可明显改善肾间质纤维化模型大鼠病情,其作用机制可能与介导肾脏组织TGF-β_1、Smad2、Smad7有关。Objective:To evaluate the therapeutic effects of Shenfukang on fibrosis model rats and to observe its effect on the expression of TGF-β1,Smad2 and Smad7 in renal tissues.Methods:A total of 70 SD rats were randomly divided into sham operation group,Shenfukang group and model group.The rats in both Shenfukang group and model group were treated with renal interstitial fibrosis model.Rats receiving different doses of Shenfukang gavage on the 2 nd day after building the model.Model group and sham operation group received equal volume of saline gavage,and both group were given intervention for 21 days.At the end of the intervention,the urine of each group was collected for 24 h,and the urinary protein in 24 h(24 UP),serum creatinine(SCr),blood urea nitrogen(BUN)levels were detected.The samples were collected after being decapitated.HE staining was used to evaluate the pathological changes of renal tissue in each group,immunohistochemical method was used to detect the changes of TGF-beta 1 protein in renal tissue in rats,Western blotting method was used to detected Smad2,Smad7 concentration.Results:1)Compared with the sham operation group,the levels of 24 UP,SCr and BUN in the Shenfukang group and model groups significantly increased(P<0.05).Compared with the model group,the level of 24 UP,SCr and BUN decreased(P<0.05),and with the increasing dose of Shenfukang the trend was more obvious.2)Compared with the sham operation group,the renal tissue pathological score of Shenfukang group and model group were significantly increased,among which the pathological score of Shenfukang group was lower than the model group(P<0.05),and the pathological score decreased gradually with the increase of the dosage of Shenfukang.3)There was little expression of TGF-β1 and Smad2 in renal tissue of the sham operation group and high expression of TGF-β1 and Smad2 in the model group.The concentration of TGF-β1 and Smad2 decreased gradually with the increase of Shenfukang concentration.After modeling,the concentration of Smad7 decreased,an

关 键 词：肾纤维化 肾复康 大鼠 肾功能 转化生长因子-β1 SMAD2 SMAD7 作用机制 

分 类 号：R289.5[医药卫生—方剂学]