蒙医瘟疫热症经典方查干汤对小鼠脾淋巴细胞增殖及白细胞介素-2和干扰素-γ分泌的影响  

Effects of Classic Mongolia Medicine Prescription Chagan Decoction for the Treatment of Plague Heat Syndrome on Spleen Lymphocyte Proliferation and Secretion of IL-2 and IFN-γ

作　　者：伊丽娜 张屏 张烜 昌妍希 杭凌宇 包保全 Yi Lina;Zhang Ping;Zhang Xuan;Chang Yanxi;Hang Lingyu;Bao Baoquan(College of Pharmacy,Inner Mongolia Medical University,Hohhot 010100,China;College of Basic Medical Sciences, Inner Mongolia Medical University,Hohhot 010100,China)

机构地区：[1]内蒙古医科大学药学院,呼和浩特010100 [2]内蒙古医科大学基础医学院,呼和浩特010100

出　　处：《世界中医药》2018年第12期3114-3117,共4页World Chinese Medicine

基　　金：国家自然科学基金项目(81460651);内蒙古蒙医药协同创新培育中心科研项目(MYYXT201506);内蒙古自治区留学回区人员开展科技活动项目择优资助重点项目

摘　　要：目的:研究蒙医瘟疫热症经典方查干汤对小鼠脾淋巴细胞增殖及诱生细胞因子白细胞介素-2和干扰素-γ的影响。方法:选取CCK-8法检测不同浓度药物对小鼠脾淋巴细胞增殖的影响;用酶联免疫吸附试验(ELISA)法检测白细胞介素-2和干扰素-γ的含量。用查干汤不同极性提取物,4个组方药材土木香、苦参、悬钩子木、山柰以及6个指标性成分土木香内酯、异土木香内酯、苦参碱、氧化苦参碱、肉桂酸乙酯、对甲氧基桂皮酸乙酯评价脾淋巴细胞增殖活性。结果:复方及组方药材土木香、苦参、悬钩子木和山柰水提物,均能不同程度促进脾淋巴细胞增殖,并诱导白细胞介素-2和干扰素-γ分泌,复方、组方药材醇提物及指标性成分,均无此作用。结论:查干汤及其组方药材水提物均能明显促进脾淋巴细胞增殖,并通过分泌白细胞介素-2和干扰素-γ发挥免疫调节作用。Objective:On the level of compound,prescription medicine,extract and index components,to study the effects of classic Mongolia medicine prescription Chagan Decoction on rat spleen lymphocyte proliferation and cell factor of IL-2 and IFN-γ.Methods:CCK-8 method was used to test the effect of different degree of drug on rat lymphocyte proliferation.The content of IL-2 and IFN-γwere tested by ELISA method.Different polar extracts of Chagan Decoction,4 composition medicinal materials including Inulae Radix,Sophorae Flavescentis Radix,Rubi Ramulus,Kaempferiae Rhizoma,as well as 6 index components,such as alantolactone,isoalantolactone,matrine,oxymatrine,ethyl cinnamate and ethyl-p-methoxycinnamate,were used for the evaluation of lymphocyte proliferation activity.Results:The aqueous extracts of Inulae Radix,Sophorae Flavescentis Radix,Rubi Ramulus,Kaempferiae Rhizoma could promote spleen proliferation lymphocyte in different degrees,and induce secretion of IL-2 and IFN-γ.However,compound,extracts of compound medicinal materials and index component did not show any proliferation effect.Conclusion:The aqueous extracts of Chagan Decoction and compound medicinal materials both could promote the spleen proliferation lymphocyte and play a role in immune regulation through IL-2 and IFN-γ.

关 键 词：传统蒙药 复方 查干汤 指标性成分 脾 淋巴细胞 增殖 细胞因子 白细胞介素-2 干扰素-Γ 

分 类 号：R289.5[医药卫生—方剂学]