基于一测多评法的白术饮片成分含量测定  

Quantitative Assay of Multi-components by Single Marker for Determination of Three Components in Atractylodes Macrocephala Koidz

作　　者：伍蕊嗣 刘涛 梁悦 周蔚昕 寇强 张佳 WU Ruisi;LIU Tao;LIANG Yue;ZHOU Weixin;KOU Qiang;ZHANG Jia(School of Pharmacy and Bioengineering, Chengdu University, Chengdu 610106, China)

机构地区：[1]成都大学药学与生物工程学院,四川成都610106

出　　处：《成都大学学报（自然科学版）》2018年第4期361-365,共5页Journal of Chengdu University（Natural Science Edition）

摘　　要：建立同时测定白术饮片中主要及有效成分(苍术酮、白术内酯I、白术内酯III)的一测多评法,完善其质量标准.考察了一测多评法高效液相色谱条件及供试品制备方法后,在一定线性范围内,建立白术内酯III与白术内酯I、苍术酮的相对校正因子;并对一测多评法的方法学、耐用性、系统适应性、色谱峰的定位进行考察;另外为求证一测多评法的科学性,对13批白术饮片同时采用了外标法进行成分含量测定.结果显示,白术饮片一测多评法的准确度、重复性、稳定性及精密度均较好;建立的相对校正因子重现性良好(f白术内酯Ⅰ/白术内酯Ⅲ=1. 6368、f苍术酮/白术内酯Ⅲ=0. 3049);相对保留值较相对时间差能更好地定位色谱峰,RSD <2%;一测多评法和外标法两法所得数值间RSD <5%.实验表明,一测多评法可作为一种简便快速的质量评价方法用于白术饮片的多成分定量分析.The paper is going to establish a quantitative assay of multi-components by single marker(QAMS) for the determination of Atractylenolide Ⅲ,Atractylenolide Ⅰ,Atractylone in Atractylodes macrocephala Koidz at the same time and meanwhile to improve the quality standards of this method.The HPLC chromatography conditions and the preparation methods of the samples are investigated.The relative correction factors of Atractylenolide Ⅲ and Atractylenolide Ⅰ,and Atractylone are established in a certain linear range.The methodology,durability,system suitability and location of chromatographic peaks are also investigated.At the same time,the content of the components in 13 batches of Atractylodes macrocephala is determined by external standard method,in order to verify the accuracy and scientificity of the quantitative assay of multi-components by single marker.The precision,stability,repeatability and accuracy of the method of measuring and evaluating Atractylodes macrocephala pieces are all good.The results show that the relative correction factor is of good reproducibility(fAtractylenolide Ⅰ/Atractylenolide Ⅲ= 1.6368;fAtractylone/Atractylenolide Ⅲ= 0.3049);the relative retention values are determined for chromatographic peaks,RSD<2% and the calculation value of QAMS method is similar to the measured value of external standard method,RSD<5%.This method can be used as a simple and rapid quality evaluation method for the quantitative analysis of multiple components of Atractylodes macrocephala Koidz.

关 键 词：一测多评法 白术 白术内酯III 质量标准 

分 类 号：R284.1[医药卫生—中药学] O657.72[医药卫生—中医学]