浙贝母原产地及其迁地引种后含量分析  

Quality Analysis of Fritillaria Thunbergii Origin and Exsitu Introduction

作　　者：雨田[1] 练心洁 孙佑玲 刘凤 张艳容 周浓 YU Tian;UAN Xinjie;SUN Youling;LIFeng;ZHANG Yanrong;ZHOU Nong(School of Nursing, Qiengdu University, Chengdu 610106, China;School of Phaitnacy and Bioengineering, Chengdu University, Chengdu 610106, China;College df Biology and Food Engineering, Chongqing Three Gorges University, Chongqing 404000, China)

机构地区：[1]成都大学医学院,四川成都610106 [2]成都大学药学与生物工程学院,四川成都610106 [3]重庆三峡学院生物与食品工程学院,重庆404000

出　　处：《成都大学学报（自然科学版）》2018年第4期370-372,383,共4页Journal of Chengdu University（Natural Science Edition）

基　　金：四川省科技厅基础应用研究(2017NZ0046)资助项目

摘　　要：对浙贝母原产地及其迁地引种后进行含量分析,为筛选适合重庆地区栽培的浙贝母种源提供实验数据.采用高效液相色谱法对浙贝母药材中生物碱类指标成分进行含量分析,采用紫外—可见分光光度法测定浙贝母药材中总生物碱含量,比较原产地及其迁地引种后浙贝母药材有效成分的变化情况.结果显示,原产地及其迁地引种后浙贝母药材中贝母素甲、贝母素乙、贝母辛等生物碱类有效成分的含量存在较大差异,结合总生物碱含量的测定结果,表明原产地及其迁地引种后浙贝母药材有效成分差异较大.实验表明,浙贝母迁地栽培是可行的,其中仁川半坑、安徽花桥处浙贝母引种后生物碱类成分含量有所上升,可引种至重庆地区栽培.The paper aims to select the theoretical basis for Fritillaria Thunbergii provenance which could be planted in Chongqing,and to do quality analysis for Fritillaria Thunbergii origin and exsitu introduction.In the study,the content of test index alkaloid in Fritillaria Thunbergii was measured by HPLC method;the total content of alkaloidal was evaluated by using ultraviolet-visible spectrophotometry;then the contents of active chemical composition were used for comparison of the Fritillaria Thunbergii in origin and exsitu.The results showed that there were huge differences in the effective contents of Peimine,Peiminine and Peimisine in Fritillaria Thunbergii origin and exsitu.With the analysis of the total content of alkaloidal,the research suggested that obvious differences existed in the chemical composition in Fritillaria Thunbergii origin and exsitu introduction.It is concluded that based on the above-mentioned results,the study proves that Fritillaria Thunbergii can be cultivated on a large scale.Because the content of alkaloid in Fritillaria Thunbergii of Inchon crater and Anhui Huaqiao has increased,the Fritillaria Thunbergii can be introduced in Chongqing for cultivation.

关 键 词：浙贝母 贝母素甲 贝母素乙 贝母辛 总生物碱 质量评价 

分 类 号：R284.1[医药卫生—中药学]