基于g-C_3N_4-CeO_2复合材料的电化学生物传感器  

Bioelectrochemical Sensor Based on g-C_3N_4-CeO_2 Composites

作　　者：李晓琴 刘雅杰 曹秀芝 王娟 姚慧 LI Xiao-qin;LIU Ya-jie;CAO Xiu-zhi;WANG Juan;YAO Hui(Shenyang University of Chemical Technology,Shenyang 110142,China)

机构地区：[1]沈阳化工大学应用化学学院,辽宁沈阳110142

出　　处：《沈阳化工大学学报》2018年第4期310-317,共8页Journal of Shenyang University of Chemical Technology

基　　金：辽宁省博士启动基金项目(201501077);沈阳化工大学博士启动基金资助项目(20063209)

摘　　要：分别采用高温煅烧法和水热合成法制备g-C_3N_4材料和g-C_3N_4-CeO_2复合材料.采用傅里叶红外光谱仪(FTIR),X-射线衍射(XRD)和扫描电镜(SEM)对材料进行表征.将g-C_3N_4-CeO_2和辣根过氧化物酶(HRP)混合修饰于玻碳电极表面后,再进行电化学聚合氯金酸,构建一种新型的用于检测过氧化氢(H_2O_2)的电化学Au/g-C_3N_4-CeO_2-HRP/GCE生物传感器.实验结果表明:该传感器对H_2O_2有较好的催化响应,在H_2O_2浓度1. 0×10-5～4. 6×10-3mol/L的范围内该传感器的响应电流与浓度呈现良好的线性关系,检出限为2. 6μmol/L(S/N=3).该传感器还应用于对实际水样的检测,其加标回收率为98. 4%～101. 2%.因此,g-C_3N_4-CeO_2复合材料在电化学传感器方面有着潜在的应用前景.G-C3N4and g-C3N4-CeO2composite materials were successfully prepared by calcined and hydrothermal synthesis method.Materials were characterized by Fourier transform infrared spectroscopy(FTIR),X-ray diffraction(XRD)and Scanning electron microscopy(SEM).Before electropolymerizating Au,g-C3N4-CeO2composites material was modified onto the surface of a glass carbon electrode with horseradish peroxidase(HRP).A novel electrochemical biosensor for H2O2was constructed by Au/g-C3N4-CeO2-HRP/GCE.The modified electrode displayed a good electrocatalytic activity towards H2O2.Under optimized conditions,the linear response of the biosensor to H2O2ranged from1.0×10^-5to4.6×10^-3mol/L,with a detection limit of2.6μmol/L(S/N=3).Satisfactory detection results in real water sample were obtained by this biosensor,and the recovery rates were between98.4%~101.2%.Thus,the g-C3N4-CeO2composites material has great potential for applications in electrochemical biosensors.

关 键 词：g-C3N4-CeO2复合材料 过氧化氢 修饰电极 酶传感器 

分 类 号：O657.1[理学—分析化学] TQ123.6[理学—化学]