1株宽噬菌谱肠出血性大肠杆菌噬菌体的分离及生物学特性分析  

ISOLATION AND BIOLOGICAL CHARACTERISTICS OF LYTIC ENTEROHEMORRHAGIC ESCHERICHIA COLI BACTERIOPHAGE

作　　者：孙利厂[1] 周艳[1] 张莉莉[1] 庞茂达[1] 何涛[1] 包红朵[1] 张辉[1] 何灵尘 王冉[1] SUN Li-chang;ZHOU Yan;ZHANG Li-li;PANG Mao-da;HE Tao;BAO Hong-duo;ZHANG Hui;HE Ling-chen;WANG Ran(Jiangsu Academy of Agricultural Sciences Institute of Food Safety and Nutrition,Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology,Key Laboratory of Control Technology and Standard for Agroproduct Safety and Quality,Ministry of Agricultrue,Key Laboratory of Animal-derived Food safety of Jiangsu Province/Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China)

机构地区：[1]江苏省农业科学院农产品质量安全与营养研究所省部共建国家重点实验室培育基地-江苏省食品质量安全重点实验室农业部农产品质量安全控制技术与标准重点实验室江苏省畜禽产品安全性研究重点实验室,南京210014

出　　处：《中国动物传染病学报》2018年第6期58-63,共6页Chinese Journal of Animal Infectious Diseases

基　　金：江苏省食品质量安全重点实验室-省部共建国家重点实验室培育基地自主研究课题;江苏省基础研究计划(自然科学基金)项目(BK20160585);江苏省农业科技自主创新项目(CX(16)1060)

摘　　要：本研究从养殖场采集的污水样品中分离到宽噬菌谱肠出血性大肠杆菌烈性噬菌体V_EcoM_C1,并进行了噬菌斑、噬菌谱、形态学(透射电镜)、遗传物质、酸碱及热稳定性、一步生长曲线等生物学特性及控制养殖环境中的肠出血性大肠杆菌的污染研究。结果表明:噬菌体V_EcoM_C1呈现出典型裂解性噬菌体特征,空斑透亮,无晕环,空斑边缘整齐清晰;噬菌体V_EcoM_C1噬菌斑直径为1.0～1.5 mm;噬菌体V_EcoM_C1头部椭圆形,长径约86 nm,横径约54 nm;噬菌体V_EcoM_C1具有可收缩性尾,尾长约76 nm,直径约10 nm,头部与尾部被颈圈隔开;噬菌体V_EcoM_C1可裂解5种不同血清型肠出血性大肠杆菌,且噬菌斑透亮,具有较宽噬菌谱;噬菌体V_EcoM_C1在30℃～50℃保持高活性;噬菌体V_EcoM_C1在pH5～10稳定;噬菌体V_EcoM_C1感染宿主菌的潜伏期约10 min,爆发持续时间约60 min,平均爆发量为26,具有较高的裂解活性;噬菌体V_EcoM_C1可以控制养殖环境中的肠出血性大肠杆菌证明该噬菌体可以有效杀灭养殖环境(奶牛料槽表面)中的肠出血性大肠杆菌。综上所述,V_EcoM_C1为1株宽噬菌谱肠出血性大肠杆菌烈性噬菌体,可用于肠出血性大肠杆菌噬菌体制剂的研发。The study was aimed to separate a Enterohemorrhagic Escherichia coli Pullorum lytic phage V_EcoM_C1 with wide host range.The feces were collected from broiler farms.And the biological parameters of the lytic phage were assayed,including the transmission electron microscopy observation,determination of host range,thermal stability,pH stability,one-step growth curve drawing.The results showed that phage V_EcoM_C1 plaques were round and transparent and the sizes were about 1.0~1.5 mm in diameter.It had an elliptical head with approximately 86 nm in diameter,54 nm in transverse diameter and had a long tail with 76 nm in length,10 nm in diameter.Phage V_EcoM_C1 could lyse 5 serotypes of Enterohemorrhagic Escherichia coli.The phage was stable with a wide range of temperature(30~50℃)and pH(5~10).The one-step growth curve showed that the latent time and burst time of V_EcoM_C1 were 10 and 60 min,respectively,and the burst size was 26.In conclusion,V_EcoM_C1 is a Enterohemorrhagic Escherichia coli Pullorum lytic phage with wide host range that could serve as a promising phage preparation against Enterohemorrhagic Escherichia coli.

关 键 词：肠出血性大肠杆菌 噬菌体 分离 生物学特性 

分 类 号：S852.612[农业科学—基础兽医学]