体重波动对小鼠血糖水平的影响  

Effects of weight fluctuation on blood glucose levels in mice

作　　者：宗艾伦 周迎生[1] ZONG Ailun;ZHOU Yingsheng(Department of Endocrinology and Metabolism, Beijing Anzhen Hospital, Capital Medical University,Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing 100029, China)

机构地区：[1]首都医科大学附属北京安贞医院内分泌代谢科,北京市心肺血管疾病研究所,北京100029

出　　处：《中国实验动物学报》2018年第6期721-726,共6页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金(81641027);北京市卫生系统高层次卫生技术人才项目(2013-2-006);北京市科委科研基金资助项目(Z131100004013044);北京市保健科研课题项目(京15-11号)~~

摘　　要：目的观察体重增减影响血糖水平变化的规律特点。方法 8周龄雄性C57BL/6小鼠高热量饲料(脂肪热量占60%)喂养8周呈肥胖模型,随机分入体重干预组(intervention group, IT)和肥胖组(obesity group,OB),肥胖组维持高热量饲料至终点。正常对照组(control group,CON)喂食普通饲料。限制热量摄入使IT组体重先减至CON组水平(P> 0.05),后恢复高热量饲料再次诱导体重反弹至OB组水平(P> 0.05)时终止。采用腹腔注射葡萄糖耐量试验反映葡萄糖代谢水平,胰岛素耐受试验评价胰岛素敏感性。胰腺病理HE染色观察胰岛形态学改变。结果 IT组体重反弹时体重增速是干预前的2.27倍,空腹血糖则高达5.13倍。与OB组相比,实验终点葡萄糖耐量试验血糖曲线下面积显著增加(P<0.05),胰岛素敏感性显著下降(P<0.05),内脏脂肪增加(P<0.05),胰岛面积显著减小(P<0.05)。结论体重波动导致内脏脂肪量增加、胰岛素敏感性降低和葡萄糖耐量受损。Objective To observe the dynamic blood glucose levels caused by diet-induced weight gain or loss.Methods Eight-week-old male C57BL/6mice were fed a high-calorie diet(60%fat)for8weeks,and then divided into an intervention group(IT)and an obesity group(OB).Mice in the OB group were fed a high-calorie diet throughout the study.A normal control group(CON)was also established and given normal chow.Limiting caloric intake reduced the weight of the IT group to that of the CON level(P>0.05),after that resuming high-calorie feeding until the body weight rise back to the OB level(P>0.05).Intraperitoneal glucose tolerance test(IPGTT)was performed to evaluate blood glucose metabolism,insulin tolerance test(ITT)for insulin sensitivity,and HE staining of the pancreas for islet morphological study.Results When IT group mice were again exposed to a high-calorie diet,body weight growth and fasting blood glucose rate were2.27times and5.13times,higher than those before caloric restriction.At the end of the intervention,larger area under the blood glucose curve of IPGTT(P<0.05),decreased insulin sensitivity(P<0.05),larger body fat depots(P<0.05),and smaller islet area were detected in comparison with those in the OB group.Conclusion Body weight fluctuations lead to an increased intraperitoneal fat mass,decreased insulin sensitivity,and impaired glucose tolerance.

关 键 词：肥胖 减重 血糖 胰岛素敏感性 

分 类 号：Q95-33[生物学—动物学]