Ag/TiO_2复合纳米材料对互隔交链孢霉的抗菌作用与机制  

Antifungal Effect and Mechanism of Ag/TiO_2 Manomaterials on Alternaria Alternata

作　　者：马一禾[1] 肖小军[1] 袁如意 陈仕国[2] 刘志刚[1] MA Yi-he;XIAO Xiao-jun;YUAN Ru-yi;CHEN Shi-guo;LIU Zhi-gang(Institute of Allergy and Immunology,Shenzhen University,Shenzhen 518060,China;College of Materials,Shenzhen University,Shenzhen 518060,China)

机构地区：[1]深圳大学过敏反应与免疫学研究所,广东深圳518060 [2]深圳大学材料学院,广东深圳518060

出　　处：《南昌大学学报（医学版）》2018年第5期12-16,共5页Journal of Nanchang University：Medical Sciences

基　　金：广东省科技计划项目(2014B090901041;2017B020226006);深圳市孔雀团队项目(KQTD20170331145453160);深圳市基础研究学科布局项目(JCYJ20160328144536436)

摘　　要：目的合成Ag/TiO2复合纳米材料,探讨其对互隔交链孢霉抗真菌作用及机制。方法采用光催化还原法制备Ag/TiO2复合纳米材料,并通过扫描电镜对其进行表征测定。通过微量液基稀释法检测Ag/TiO2复合纳米材料对互隔交链孢霉的最低抑菌质量浓度,液体振荡培养检测其对真菌生物量的抑制作用,Elisa试剂盒以及荧光显微镜下检测其具体抗真菌作用机制。结果 Ag/TiO2复合纳米材料合成成功,电镜可观察到其分布均匀、大小均一。对互隔交链孢霉具有较强的杀灭作用,最低抑菌质量浓度为16μg·mL-1,且在该最低抑菌质量浓度下可对真菌生物量的抑制作用达到100%。主要通过降低真菌体内谷胱甘肽还原酶和还原型谷胱甘肽等抗氧化物质的含量,从而破坏真菌机体抗氧化防御系统,并诱导体内产生过量活性氧,同时伴随着线粒体膜电位的下降,导致真菌细胞的凋亡。结论 Ag/TiO2复合纳米材料对气传真菌孢子和菌丝在空气传播中的阻断作用具有重要意义。Objective To investigate the antifungal effect and mechanism of Ag/TiO2composite nanomaterials against Alternaria alternata.Methods Ag/TiO2nanomaterials were prepared by photocatalytic reduction,and their characterization was determined by scanning electron microscopy.The minimum inhibitory concentration of Ag/TiO2nanomaterials was detected by microdilution method.The inhibitory effect of Ag/TiO2nanomaterials on fungal biomass was measured by oscillatory liquid culture.ELISA kit and fluorescence microscopy were used to analyze the specific antifungal mechanism.Results The Ag/TiO2composite nanomaterials were successfully synthesized with uniform distribution and size.They had a strong antifungal effect on Alternaria alternata,and the inhibition on fungus biomass reached100%at the minimum inhibitory concentration(16μg·mL-1).The Ag/TiO2composite nanomaterials destroyed anti\|oxidative defense system and induced excessive reactive oxygen production by reducing the content of antioxidants such as glutathione reductase and glutathione in the fungus.Meanwhile,they decreased mitochondrial membrane potential,and therefore led to the apoptosis of fungal cells.Conclusion Ag/TiO2composite nanomaterials are of great significance for blocking the propagation of airborne fungal spores and mycelia.

关 键 词：Ag/TiO2复合纳米材料 互隔交链孢霉 表征测定 抗真菌作用与机制 

分 类 号：R379[医药卫生—病原生物学]