不同燕麦品种耐盐性差异及其生理机制  

Evaluation of salt tolerance and its underlying physiological mechanisms in different oats

作　　者：姜瑛[1] 周萌 吴越 苗玉红[1] 汪强[1] 韩燕来[1] JIANG Ying;ZHOU Meng;WU Yue;MIAO Yuhong;WANG Qiang;HAN Yanlai(College of Resources and Environmental Sciences, Henan Agricultural University,Zhengzhou 450000, Henan, China;Agricultural Economy and Information Research Institution,Henan Academy of Agricultural Sciences, Zhengzhou 450008, Henan, China;Soil and Fertilizer Bureau of Shandong Province, Jinan 250100, Shandong, China)

机构地区：[1]河南农业大学资源与环境学院,河南郑州450000 [2]河南省农业科学院农业经济与信息研究所,河南郑州450008 [3]山东省土壤肥料总站,山东济南250100

出　　处：《草业科学》2018年第12期2903-2914,共12页Pratacultural Science

基　　金："十二五"农村领域国家科技计划项目"砂质潮土中低产田改良研究与示范"(2012BAD05B0207)

摘　　要：在水培条件下,以加拿大引进燕麦(Avena sativa)品种加1(Tybor naked oat)、加8(Marion oat 2002)、加9(Nova oat 2003)和白燕6号为试验材料,从盐害外观症状、生理指标及离子吸收方面综合评价不同基因型燕麦对盐胁迫浓度和胁迫时间的生理适应性。结果表明,各燕麦品种不同盐浓度处理间叶绿素含量、丙二醛含量、脯氨酸含量、Na+、K+吸收及K+/Na+达到差异显著水平(P<0.05)。随着胁迫浓度的升高和时间的延长,4个品种盐害症状逐渐明显且有逐渐加重的趋势,叶绿素含量逐渐降低,丙二醛含量不断增加,Na+含量增加而K+含量降低,整体K+/Na+呈现降低的趋势。加8的盐害指数最低、叶绿素含量降低较少、丙二醛积累相对较低,脯氨酸累积能力强且具有较高的K+/Na+,耐盐能力综合评价值最高;白燕6号在中高盐胁迫下盐害指数稍高于前者、叶绿素含量降低较少、丙二醛积累相对较低,在中高盐浓度下脯氨酸积累能力强,但K+/Na+较低,耐中高盐胁迫下能力次之;加1和加9在中高盐胁迫下盐害指数较高、叶绿素含量降低显著、丙二醛积累较高,脯氨酸积累能力相对弱,且降低较早,同时K+/Na+较低,对盐胁迫相对较敏感。研究表明,加8耐盐性高于其他品种的主要原因在于盐胁迫下有着较强的持K+能力,能够保持较高的K+/Na+,而白燕6号耐盐性强可能与其在Na+区隔化或脯氨酸渗透调节能力具有一定的优势有关。A hydroponic experiment was carried out to investigate the salt tolerance in four different oat varieties(Tybor naked oat,Marion oat2002,Nova oat2003,and Baiyan6)under various salt concentrations and retention times.The subjective symptoms,physiological index,and ion adsorption by the plants were recorded.Results showed that the chlorophyll,malondialdehyde,and proline content,as well as absorption of Na^+,K^+,and corresponding K^+/Na^+ratios in the different oat varieties were significantly different at a0.05significance level.Salt injury occurred at increasing salt concentrations and retention times,whereby the plants exhibited decreasing chlorophyll concentrations,increasing malondialdehyde concentrations,and low ratios of K^+/Na^+.In summary,Marion oat2002had the highest salt tolerance among the four varieties,and thus,exhibited lowest decrease in chlorophyll concentration,as well as relatively lower malondialdehyde and higher proline accumulations;it also had higher ratios of K^+/Na^+and the lowest salt injury index.Baiyan6had the second highest salt tolerance,which manifested in the form of a lower decrease in chlorophyll concentration,as well as relatively lower malondialdehyde and higher proline accumulations;there was a lower ratio of K^+/Na^+.Compared to Marion oat2002and Baiyan6,Tybor naked oat and Nova oat2003had the lowest salt tolerance with a significant decrease in chlorophyll concentration,relatively higher malondialdehyde and lower proline accumulations,and lower ratios of K^+/Na^+.These results indicate that the main reason for the higher salt tolerance of Marion oat2002than other varieties was its stronger ability to absorb K^+and maintain higher ratios of K^+/Na^+under salt stress,while the reason for salt tolerance in Baiyan6may be attributable to its ability to compartmentalize Na^+in its vacuoles and its stronger ability to adjust proline.

关 键 词：燕麦 盐胁迫 脯氨酸 丙二醛 叶绿素 盐害指数 隶属函数值 

分 类 号：S512.603.4[农业科学—作物学] Q945.78[生物学—植物学]