激素对不同种源樟树体胚发生的影响  

Effects of hormon on somatic embryogenesis from different provenances of Cinnamomum camphora

作　　者：杨柳 李志辉[1] 林立彬 YANG Liu;LI Zhihui;LIN Libin(College of Forestry, Central South University of Forestry and Technology, Changsha 410004, Hunan, China)

机构地区：[1]中南林业科技大学林学院,湖南长沙410004

出　　处：《中南林业科技大学学报》2018年第12期115-120,135,共7页Journal of Central South University of Forestry & Technology

基　　金："十三五"国家重点研发计划项目"樟树等高效培育技术研究"(2016YFD06006005)

摘　　要：基因研究依赖于植株再生,香樟离体培养植株再生以体胚发生为主。以岳阳、郴州、醴陵、长沙、怀化、娄底樟树Cinnamomum camphora未成熟合子胚作为外植体,研究不同激素在体胚发生过程中对体胚及愈伤组织诱导、芽分化和生根的影响,目的在于为湖南省不同种源樟树体胚发生建立基础,完善樟树植株再生体系。结果表明:适合岳阳、怀化樟树诱导体胚发生的最适培养基为MS+1 mg·L^(-1) 6-BA+0.1 mg·L^(-1) 2,4-D,其余种源均为MS+1 mg·L^(-1) 6-BA+0.3 mg·L^(-1) 2,4-D;岳阳、怀化、娄底种源樟树芽分化最佳培养基为MS+5 mg·L^(-1) 6-BA+0.1 mg·L^(-1) NAA,醴陵和长沙种源樟树为MS+3 mg·L^(-1) 6-BA+0.3 mg·L^(-1) NAA,郴州种源为MS+5 mg·L^(-1) 6-BA+0.3 mg·L^(-1) NAA;诱导岳阳、郴州、醴陵、长沙、怀化、娄底樟树芽生根的最适激素浓度分别为1 mg·L^(-1) IBA+0.1 mg·L^(-1) NAA、1 mg·L^(-1) IBA+0.5 mg·L^(-1) NAA、1 mg·L^(-1) IBA+0.3 mg·L^(-1) NAA、1mg·L^(-1) IBA、1 mg·L^(-1) IBA+0.1 mg·L^(-1) NAA、1 mg·L^(-1) IBA+0.3 mg·L^(-1) NAA。该研究为樟树的遗传改良和新品种培育提供重要参考依据。Gene research depended on plant regeneration,in vitro plantlet regeneration of Cinnamomum camphora was mainly embryogenesis.Explants from the immature zygotic embryos of Yueyang,Chenzhou,Liling,Huaihua,Loudi,Changsha,research different hormones in the process of embryo effects on induction,somatic embryo callus and bud differentiation and rooting,this study established the basis for the emergence of different species of camphor tree somatic embryos in Hunan Province,and improved the plant regeneration system of camphor tree.The results show that:for Yueyang,Huaihua camphor induced somatic embryogenesis of the optimum culture medium was MS+1mg·L^-16-BA+0.1mg·L^-12,4-D,the other provenances were MS+1mg·L^-16-BA+0.3mg.·L^-12,4-D;from Yueyang,Huaihua,Loudi,camphor tree bud differentiation medium were the best for MS+5mg·L^-16-BA+0.1mg·L^-1NAA,Liling and Changsha provenances MS+3mg·L^-16-BA+0.3mg·L^-1NAA,Chenzhou for the MS+5mg·L^-16-BA+0.3mg·L^-1NAA;Yueyang,Chenzhou,Liling,Huaihua,Loudi,Changsha camphor tree rooting optimal hormone concentrations were1mg·L^-1IBA+0.1mg·L^-1NAA,1mg·L^-1IBA+0.5mg·L^-1NAA,1mg·L^-1IBA+0.3mg·L^-1NAA,1mg·L^-1IBA,1mg·L^-1IBA+0.1mg·L^-1NAA,1mg·L^-1IBA.This study will provide important reference for genetic improvement and cultivation of Cinnamomum camphora.

关 键 词：樟树 体细胞胚 植株再生 激素 

分 类 号：S792.23[农业科学—林木遗传育种]