15-脱氧-Δ^(12,14)-前列腺素J_2对大鼠酸性胃内容物吸入性肺损伤的保护作用  

Protective effect of 15 deoxy-Δ^(12,14)-prostaglandin J_2 on a rat model of combined acid and gastric particles-induced aspiration lung injury

作　　者：施劲东 隆玄[2] 符翠萍[2] 李建楠 谢娟 施天昀 梅周芳 揭志军 李善群[2] SHI Jin-dong;LONG Xuan;FU Cui-ping;LI Jian-nan;XIE Juan;SHI Tian-yun;MEI Zhou-fang;JIE Zhi-jun;LI Shan-qun(Department of Respiratory, The Fifth People’s Hospital of Shanghai, Fudan University, Shanghai 200240, China;Department of Respiratory and Critical Care Medicine, Zhongshan Hospital, Fudan University, Shanghai 200032, China)

机构地区：[1]复旦大学附属上海市第五人民医院呼吸科,上海200240 [2]复旦大学附属中山医院呼吸与危重症医学科,上海200032

出　　处：《中国临床医学》2018年第6期905-909,共5页Chinese Journal of Clinical Medicine

基　　金：上海市卫计委科研项目(201640237);上海市科委科研项目(18411970400);复旦大学附属上海市第五人民医院卓越人才培养计划(2017WYRCZY01)~~

摘　　要：目的:探讨15-脱氧-Δ^(12,14)-前列腺素J_2(15-deoxy-Δ^(12,14)-prostaglandin J2,15d-PGJ_2)在大鼠酸性胃内容物(combined acid and gastric particles,CAGP)吸入性肺损伤中的保护作用。方法:制备大鼠CAGP模型,将20只大鼠模型随机分为对照组与治疗组,每组10只。对照组:气管内滴注CAGP,腹腔内注射PBS;治疗组:气管内滴注CAGP,腹腔内注射15dPGJ_2。肺损伤后4h和12h,两组各取5只大鼠采集标本,测定动脉血氧分压(PaO_2)、肺组织湿重/干重比值(W/D)、支气管肺泡灌洗液(bronchoalveolar lavage fluid,BALF)总蛋白浓度、BALF白细胞计数和分类计数、BALF和血清肿瘤坏死因子-α(TNF-α)浓度、肺组织苏木精-伊红(H-E)染色炎症评分等指标。结果:与对照组比较,损伤后4h和12h,治疗组大鼠肺组织H-E染色炎症程度减轻,肺组织炎症评分下降,动脉血压分压(PaO_2)升高,肺组织W/D、BALF总蛋白浓度、白细胞总数和中性粒细胞数均下降,BALF和血清TNF-α浓度下降(P<0.05)。结论:15d-PGJ_2能减轻大鼠CAGP吸入性肺损伤和肺部炎症水平,改善动脉血氧分压。Objective:To investigate the protective effect of15d-PGJ2treatment on a rat model of combined acid and gastric particles(CAGP)-induced aspiration lung injury.Methods:The model of CAGP-induced aspiration lung injury model was established in SD rats.Twenty SD rats were randomly divided into control group and experimental group,with10rats in each group.Control group:rats were treated with CAGP intratracheal instillation and PBS intraperitoneal injection;experimental group:rats were treated with CAGP intratracheal instillation and15d-PGJ2intraperitoneal injection.At4h and12h after CAGP instillation,the arterial partial pressure of oxygen(PaO2),wet-to-dry weight ratio(W/D)of lung tissue,total protein concentration in bronchoalveolar lavage fluid(BALF),white blood cell count and differential count in BALF,tumor necrosis factor-α(TNF-α)concentration in BALF and serum,and lung inflammation score by H&E staining were analyzed in samples of5rats from each group.Results:Compared with control group,inflammatory scores based on histology were alleviated,and PaO2was improved at4h and12h after CAGP injury in15d-PGJ2treatment group.Compared with control group,W/D of of lung tissue,total protein concentration in BALF,white blood cell count and differential count in BALF,TNF-αconcentration in BALF and serum were decreased at both timepoints in15d-PGJ2treatment group(P<0.05).Conclusions:15d-PGJ2could alleviate the levels of lung injury and inflammation,and improve the PaO2in rat CAGP-induced aspiration lung injury models.

关 键 词：15-脱氧-Δ12 14-前列腺素J2 酸性胃内容物 吸入性肺损伤 

分 类 号：R563.1[医药卫生—呼吸系统] R-332[医药卫生—内科学]