海底重燃油对海胆繁殖及其子代发育的影响  

Exposure of adult sea urchins to sunken heavy fuel oil affects the reproductive status and the development of their offspring

作　　者：段美娜[1] 刘泳江 白雪 高翔[1] 张欣欣 熊德琪[1] DUAN Mei-na;LIU Yong-jiang;BAI Xue;GAO Xiang;ZHANG Xin-xin;XIONG De-qi(Department of Environmental Science and Engineering,Dalian Maritime University,Dalian 116026,China;Technology of Oily Sludge Eco-Treatment,Dalian 116000,China)

机构地区：[1]大连海事大学环境科学与工程学院,辽宁大连116026 [2]中科海创环境科技(大连)有限公司,辽宁大连116000

出　　处：《中国环境科学》2018年第12期4720-4729,共10页China Environmental Science

基　　金：国家自然科学基金资助项目(41276105)

摘　　要：利用室内流水式粘油砾石柱模拟实际环境中的海底重燃油,研究了重燃油污染的孔隙水对成年海胆繁殖力、配子质量及子代胚胎发育的影响.结果表明,暴露结束后(7d),暴露组海胆的排配子率显著降低(P=0.033),同时雌海胆繁殖力也显著降低(P=0.036,(1957917±811471)个卵细胞);卵细胞的直径和精子的受精能力并未受到海底重燃油的影响.子代继续暴露48h,发现亲代暴露加剧了子代胚胎畸形程度,表明亲代暴露的毒性可传递给子代.进一步利用整合毒性指数(ITI)检测毒性传递的性别差异发现,与母本效应相比(24和48h子代的ITI分别为0.54～1.45和1.1～2.57),父本效应(24和48h子代的ITI分别为0.82～1.95和1.89～4.04)在毒性传递过程中起着关键作用.The present study investigated effects of exposure to sunken heavy fuel oil(HFO)on the fecundity,gamete quality and development of the offspring in the sea urchin Strongylocentrotus intermedius.Adult sea urchins were exposed to effluents from HFO-oiled gravel columns for7days to simulate an oil contaminated gravel shore.The spawning ability of adults and the fecundity((1957917±811471)eggs)of females significantly decreased(P=0.033and P=0.036,respectively).No effect was observed on the egg size and fertilization success.However,a significant increase in the percentage of abnormality of the offspring were observed,demonstrating that parental exposure(especially paternal exposure)had adverse effects on the offspring.The offspring from exposed fathers showed higher ITI values(ITI values of24and48h offspring were0.82~1.95and1.89~4.04,respectively)with a higher number of malformed embryos compared to maternal exposure(ITI values of24and48h offspring were0.54~1.45and1.1~2.57,respectively),indicating that detrimental effects of sunken HFO on the early development of sea urchin embryos may be largely attributed to paternal effects.

关 键 词：海底重燃油 孔隙水 亲本效应 海胆 早期发育 

分 类 号：X55[环境科学与工程—环境工程]