视黄酸诱导基因-Ⅰ在家禽天然免疫应答中作用的研究进展  

Research Progress on Role of Retinoic Acid Inducible Gene-Ⅰ in Innate Immune Response of Poultry

作　　者：李雪峰 任梦婷 许丽文[1] 王芳芳[1] LI Xuefeng;REN Mengting;XU Liwen;WANG Fangfang(College of Animal Science and Technology , Northeast Agricultural University,Harbin 150030, China)

机构地区：[1]东北农业大学动物科学技术学院,哈尔滨150030

出　　处：《中国畜牧兽医》2018年第12期3479-3485,共7页China Animal Husbandry & Veterinary Medicine

基　　金：黑龙江省应用技术研究与开发计划项目(PC13S02)

摘　　要：家禽的天然免疫应答在抵抗病毒感染的过程中起着关键性作用,视黄酸诱导基因-Ⅰ(retinoic acid inducible gene-Ⅰ,RIG-Ⅰ)作为细胞质内一类识别病毒双链RNA的模式识别受体,与天然免疫应答密切相关。它可通过RNA配体结合病原相关分子模式监测细胞质中的病毒RNA,此过程激活了RIG-Ⅰ及下游线粒体抗病毒信号蛋白(MAVS),最终导致干扰素调节因子(IRF3/7)和核因子κB(NF-κB)活化,诱导产生Ⅰ型干扰素等免疫细胞因子,进而使细胞做出相应的抗病毒天然免疫反应。但由于鸡体内缺乏RIG-Ⅰ基因,目前大多将鸭源或鹅源RIG-Ⅰ基因转染鸡成纤维母细胞(DF-1)研究RIG-Ⅰ基因在鸡感染禽类病毒时是否具有免疫功能。文章介绍了RIG-Ⅰ在家禽体内的表达及其介导的抗病毒天然免疫信号通路,并简述了RIG-Ⅰ在家禽体内抗病毒作用的研究概况,为抑制家禽病毒的感染和免疫系统研究,以及研制新型抗病毒疫苗或免疫佐剂等提供参考。The innate immune response of poultry plays a key role in protecting the host from viral infections.Retinoic acid inducible gene-Ⅰ(RIG-Ⅰ)is used as a class of cytoplasmic internal recognition receptor of viral double-stranded RNA which is closely related to the natural immune response.It can monitor viral RNA in the cytoplasm by RNA ligand binding to pathogen-associated molecular patterns,and that process can activate RIG-Ⅰ and downstream mitochondrial antiviral signaling protein(MAVS),eventually leading to the activation of interferon regulatory factor(IRF3/7)and NF-κB,induce the production of type Ⅰinterferon and other immune cytokines,and then the cells make a corresponding anti-viral natural immune response.However,due to the lack of RIG-Ⅰ gene in chickens,the current researches are most to transfer RIG-Ⅰ gene from duck or geese source to chicken fibroblasts(DF-1)to study whether RIG-Ⅰ gene has immune function in chicken infected avian viruses.This article describes the expression of RIG-Ⅰin poultry and its mediated antiviral innate immune signaling pathway,outlines the research status of antiviral effects of RIG-Ⅰin poultry to provide references in the study of infection and immune system of poultry and the development of new anti-virus vaccines and immune adjuvants.

关 键 词：视黄酸诱导基因-Ⅰ(RIG-Ⅰ) 家禽 天然免疫应答 抗病毒 

分 类 号：S852.4[农业科学—基础兽医学]