猪繁殖与呼吸综合征活疫苗与LT蛋白配伍经黏膜途径接种小鼠免疫效果分析  

Analysis of immune effect of vaccine against porcine reproductive and respiratory syndrome virus(PRRSV) combined with LT protein through mucosal system

作　　者：李本强[1,2] 陶洁 程靖华[1,2] 马玉飞 刘雷[1] 刘惠莉 LI Ben-qiang;TAO Jie;CHENG Jing-hua;MA Yu-fei;LIU Lei;LIU Hui-li(Institute of Animal Science and Veterinary Medicine,Shanghai Academy of Agricultural Sciences;Shanghai Engineering Research Center for Breeding Pigs,Shanghai 201106,China;Shanghai key laboratory of Agri-Genetics and Breeding,Shanghai 201106,China)

机构地区：[1]上海市农业科学院畜牧兽医研究所,上海种猪工程技术研究中心,上海201106 [2]上海市农业遗传育种重点实验室,上海201106

出　　处：《上海农业学报》2018年第6期55-59,共5页Acta Agriculturae Shanghai

基　　金：动物重大疫病新型广谱中和抗体疫苗研发(国家重大研发计划项目动物重大疫病新概念防控产品研发)2017YFD0501102;上海市科技兴农重点攻关项目[沪农科攻字(2013)第3-6];上海市产业体系项目[沪农科产字(2017)第6号];上海市农口青年成长计划项目(沪农青2016第1-35号)

摘　　要：旨在评价猪繁殖与呼吸综合征(Porcine reproductive and respiratory syndrome,PRRS)活疫苗与大肠杆菌热敏性肠毒素(LT)蛋白配伍后经滴鼻途径接种小鼠后的免疫应答水平,分析猪繁殖与呼吸综合征活疫苗经黏膜途径免疫的可行性。试验共分为四组,分别为对照组、PRRSV滴鼻组、PRRSV+LT滴鼻组和PRRSV注射组。一免、二免后检测血清Ig G和鼻拭子Ig A抗体水平,同时检测IFN-γ和IL-10细胞因子的表达。结果表明:二免后,PRRSV+LT滴鼻组可产生较高水平的Ig G抗体,与PRRSV注射组无显著差异; PRRSV+LT滴鼻组产生的Ig A抗体水平明显高于PRRSV注射组; PRRSV+LT滴鼻组小鼠脾脏IFN-γ、IL-10水平与PRRSV注射组无显著差异。因此,猪繁殖与呼吸综合征活疫苗与LT蛋白经滴鼻途径免疫小鼠,可产生理想的黏膜免疫应答及系统的体液免疫和细胞免疫反应。黏膜分泌性抗体的产生,为阻断病毒局部感染提供了保护屏障,能更有效达到抵抗病毒入侵及感染的目的。Object of this paper is to evaluate the immune response to the combined use of PRRSV vaccine and E.coli heat-labile enterotoxin(LT)protein through intranasal inoculation of mice and further verify the feasibility of mucosal immunity induced by PRRSV vaccine.Four groups of subjects were included:the control,nasal inhalation of PRRSV,nasal inhalation of PRRSV+LT and injection of PRRSV.IgA in throat swab,IgG in serum and the expression of IFN-γand IL-10 in spleen of mice were measured after first and second immunity.The results showed that after second immunity,higher IgG level were detected in vaccine immunized mouse with LT adjuvant,showing no significant difference compared with injection immunization group.However,under vaccine immunized with LT adjuvant,IgA level in intranasal immunization group was significantly higher than injection immunization group.There were no significant difference between intranasal group and injected group in splenetic IFN-γand IL-10 levels.The paper concludes that PRRSV vaccine combining with LT adjuvant can result in preferable mucosal immunity by means of nasal inhalation and boost systematic humoral and cellular immunity.The generation of mucosal secretory antibody were protective to local virus infection,giving rise to more efficacious resistance to virus invasion.

关 键 词：LT佐剂 猪繁殖与呼吸综合征 滴鼻免疫 

分 类 号：S852.[农业科学—基础兽医学]