重组酶聚合酶扩增技术的研究进展及其应用  

Advances and applications of recombinase polymerase amplification

作　　者：杜亚楠 赵笑[2,3] 范小瑞 张琪 赵凯 许燕[1] DU Ya-nan;ZHAO Xiao;FAN Xiao-rui;ZHANG Qi;ZHAO Kai;XU Yan(College of Life and Environment Sciences,Shanghai Normal University,Shanghai 200234,China;Biotechnology Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai 201106,China;Shanghai Key Laboratory of Agricultural Genetics and Breeding,Shanghai 201106,China)

机构地区：[1]上海师范大学生命与环境科学学院,上海200234 [2]上海市农业科学院生物技术研究所,上海201106 [3]上海市农业遗传育种重点实验室,上海201106

出　　处：《上海农业学报》2018年第6期117-122,共6页Acta Agriculturae Shanghai

基　　金：上海市科学技术委员会科研计划项目(17391901800)

摘　　要：重组酶聚合酶扩增技术(RPA)是一种新型的恒温核酸扩增技术。本文介绍了RPA技术的扩增原理、引物设计、成本分析及产物检测等内容,归纳了RPA技术与其他等温扩增方法的优缺点,并对RPA技术在病原微生物、基因突变和食品安全检测中的应用现状进行了阐述。希望RPA技术可以得到更多生物学家的关注,更好地应用于生命科学的各个领域。Recombinase polymerase amplification(RPA)is a novel isothermal nucleic acid amplification technology.This paper introduces the RPA amplification principle,primer design,cost analysis,product detection and so on,concludes the advantages and disadvantages of RPA and other isothermal amplification technology,and expounds the present application in pathogenic microorganisms,mutation and food safety inspections.It is expected that RPA technology can draw more attention from biologists and be better applied in all fields of life science.

关 键 词：重组酶聚合酶扩增 核酸检测 引物设计 产物检测 研究进展 

分 类 号：Q78[生物学—分子生物学]