人参皂苷Rh2抑制致龋菌生物膜的实验研究  

Study on Inhibitory Effect of Ginsenoside Rh2 on Cariogenic Bacteria Biofilms

作　　者：曹茜茜[1] 叶倩琳 周立波[2] 樊明文[1] 刘畅[1] Cao Xi-xi;Ye Qian-lin;Zhou Li-bo;Fan Ming-wen;Liu Chang.(The State Key Laboratory Breeding Base of Basic Science of Stomatology(Hubei-MOST)&Key Laboratory of Oral Biomedicine Ministry of Education,School&Hospital of Stomatology,Wuhan University,Wuhan430079,China;School&Hospital of Stomatology,Jiamusi University,Jiamusi154000,China.)

机构地区：[1]口腔基础医学省部共建国家重点实验室培育基地和口腔生物医学教育部重点实验室,武汉大学口腔医学院,湖北武汉430079 [2]佳木斯大学口腔医学院,黑龙江佳木斯154000

出　　处：《口腔医学研究》2018年第12期1302-1306,共5页Journal of Oral Science Research

基　　金：国家自然科学基金(编号:81201260);湖北省自然科学基金(编号:2018CFB486).

摘　　要：目的:通过体内外实验探究人参皂苷Rh2对致龋生物膜的作用。方法:运用结晶紫染色法和激光共聚焦扫描显微镜观察Rh2对体外生物膜的影响。建立大鼠龋齿模型,取颌骨进行显微断层扫描,Keyes龋齿计分,评价Rh2的抗龋作用。结果:体外实验结果表明Rh2能明显抑制生物膜上致龋菌生长,减少胞外基质。动物实验中,Rh2组磨牙体积和窝沟区域矿物密度大于对照组(P<0.05)。Rh2组磨牙龋齿计分都低于对照组(P<0.05)。结论:Rh2能在体外抑制致龋生物膜形成,体内有一定的抗龋保护能力。Objective:To explore the effect of ginsenoside Rh2on cariogenic biofilm in vivo and in vitro.Methods:Crystal violet staining and laser scanning confocal microscopy were used to observe the effect of Rh2on biofilm in vitro.A dental caries rat model was established.Microtomographic examination of the jaw bone and Keyes caries score were used to evaluate the protective effect of Rh2on dental caries.Results:In vitro experiments showed that Rh2significantly inhibited the growth of cariogenic bacteria on the biofilm and reduced the extracellular matrix.In animal experiments,the volume and density of pit area in the molars of group Rh2rats were higher than those of the control group(P<0.05).The caries scores of the molar in group Rh2rats were lower than that in the control group(P<0.05).Conclusion:Rh2can inhibit cariogenic biofilm formation in vitro and protect rats from dental caries.

关 键 词：人参皂苷 生物膜 变形链球菌 茸毛链球菌 血链球菌 

分 类 号：R285[医药卫生—中药学]