东海产麻痹性贝毒链状亚历山大藻共附生菌群多样性研究  

Biodiversity study of the bacterial community associated with toxic marine dinoflagellate Alexandrium catenella LZ1706

作　　者：唐莹莹 乔玉宝 蒋志伟 张静 张若男 田晓清[1] 马丽艳[1] 张晓玲 陆亚男[1] 樊成奇[1] 杨桥 TANG Ying-ying;QIAO Yu-bao;JIANG Zhi-wei;ZHANG Jing;ZHANG Ruo-nan;TIAN Xiao-qing;MA Li-yan;ZHANG Xiao-ling;LU Ya-nan;FAN Cheng-qi;YANG Qiao(East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China;Shanghai Ocean University, Shanghai 201306, China;Ocean College of Marine Science and Technology, Zhejiang Ocean University, Zhoushan 316021, China;Comprehensive Technology Service Center of Zhoushan Entry-Exit Inspection and Quarantine Bureau, Zhoushan 316021, China;Center of Research in Life Sciences & Environmental Sciences, Harbin University of Commerce, Harbin 150076, China)

机构地区：[1]中国水产科学研究院东海水产研究所,上海200090 [2]上海海洋大学,上海201306 [3]浙江海洋大学海洋科学与技术学院,浙江舟山316022 [4]舟山出入境检验检疫局综合技术服务中心,浙江舟山316021 [5]哈尔滨商业大学生命科学与环境科学研究中心,黑龙江哈尔滨150076

出　　处：《海洋渔业》2018年第6期720-727,共8页Marine Fisheries

基　　金：国家自然科学基金项目(41876114,41206093);浙江省自然科学基金项目(LY18D060007);上海市科学技术委员会仪器与试剂专项(15142201600);浙江海洋大学人才引进科研基金;舟山市公益类科技项目(2017C32083);中央级公益性科研院所基本科研业务费(2014M02,2012T08)

摘　　要：有害赤潮(harmful algal bloom,HABs)是全球性的严重海洋生态灾害及重大环境热点问题,其产生的赤潮毒素经食物链传递严重威胁人类健康与生命安全。其中,麻痹性贝类毒素(paralytic shellfish poison,PSP)是已知贝类毒素中分布最广、毒性最强、对人类威胁最严重的贝毒,但PSP来源问题仍悬而未决。藻菌相互作用关系是揭示PSP产生机制的关键,为揭示产PSP典型赤潮甲藻——东海链状亚历山大藻(Alexandrium catenella LZ1706)的共附生菌群多样性信息,通过Illumina MiSeq高通量测序分析了LZ1706的共附生菌群种类及相对丰度,同时对其可培养共附生菌株进行了选择性分离及其16S rRNA基因序列扩增与系统发育分析。结果表明,链状亚历山大藻(LZ1706)共附生菌群包括6门,10纲,19目,25科及34属。其中优势门4个,包括变形菌门(Proteobacteria,53. 9%)、拟杆菌门(Bacteroidetes,27. 5%)、蓝藻门(Cyanobacteria,16. 8%)及螺旋菌门(Spirochaetae,1. 5%);优势属5个,包括Cryomorphaceae科未知属(22. 9%)、Cyanobacteria纲未知属(16. 8%)、红细菌科(Rhodobacteraceae)未知属(7. 9%)、糖螺菌属(Saccharospirillum,14. 7%)及Maricaulis属(5. 6%),其未知属种群比例高达50. 6%。分离获得的12株可培养菌株中LZ-27及LZ-7分别为Ponticoccus属与Pseudooceanicola属潜在的新种。Harmful algal blooms(HABs)is a serious global marine ecological disaster and a major hot environmental issue.Marine red-tide toxins arising from HABs cause great harm to the health of the human beings.Among the diverse red-tide toxins,paralytic shellfish poison(PSP)is the most widespread and has strong toxicity.However,the issue of the real source of PSP remains unsolved.Algae-bacterial interaction(ABI)is the key to reveal PSP production mechanism.To reveal the biodiversity of the symbiotic and epiphytic bacteria associated with the toxic dinoflagellate,Alexandrium catenella LZ1706,from the East China Sea,the species types and the relative abundance of the bacterial community were analyzed by Illumina MiSeq high-throughput sequencing,and the cultivable bacterial strains were also isolated and the phylogenetic relationship were analyzed based on16S rRNA gene sequencing.From the results obtained,the symbiotic flora included6phylum,10classes,19orders,25families and34genera,and over50.6%of the bacteria belonged to the unidentified or unknown novel genus.The results showed that the bacterial community belonged to6phylums,of which the three dominant were Proteobacteria(53.95%),Bacteroidetes(27.52%)and Cyanobacteria(16.81%).Furthermore,these6phylums were classified into34genus,while the dominant included unknown species in family Cryomorphaceae(22.86%),unknown species in Cyanobacteria(16.81%),Saccharospirillum sp.(14.65%),unknown species in family Rhodobacteraceae(7.93%)and Maricaulis sp.(5.64%).Moreover,two strains,LZ-27and LZ-7,were potential novel species of Ponticoccus sp.and Pseudooceanicola sp.,respectively,among12strains isolated by microbial pure culture method.

关 键 词：链状亚历山大藻 麻痹性贝毒 共附生菌 多样性 高通量测序 

分 类 号：S932.8[农业科学—渔业资源]