灰葡萄孢菌诱导的百合转录辅激活因子LlMBF1a基因的克隆及其特征分析  

Cloning and characterization of LlMBF1a in lily induced by gray mold pathogen

作　　者：曹兴[1] 张秀省[1] 侯栋[2] 隋娟娟[3] 穆红梅[1] 高祥斌[1] 吕福堂[1] 郭尚敬[1] 王桂清[1] CAO Xing;ZHANG Xiusheng;HOU Dong;SUI Juanjuan;MU Hongmei;GAO Xiangbin;Lv Futang;GUO Shangjing;WANG Guiqing(College of Agriculture,Liaocheng University,Liaocheng 252059,China;Vegetables Research Institute,Gansu Academy of Agricultural Sciences,Lanzhou 730070,China;College of Biology and Food Engineering,Fuyang Normal College,Fuyang 236037,China)

机构地区：[1]聊城大学农学院,山东聊城252059 [2]甘肃省农业科学院蔬菜研究所,甘肃兰州730070 [3]阜阳师范学院生物与食品工程学院,安徽阜阳236037

出　　处：《浙江农业学报》2018年第12期2024-2030,共7页Acta Agriculturae Zhejiangensis

基　　金：国家自然科学基金(31601788);山东省自然科学基金(ZR2016CB36);聊城大学博士科研启动基金(318051312)

摘　　要：转录辅激活因子MBF1a参与了植物对病原真菌的防御反应。利用电子克隆结合RT-PCR技术从百合中分离了LlMBF1a基因,其开放阅读框长429 bp,编码142个氨基酸。LlMBF1a分子量为15. 53 ku,理论等电点为10. 12,为稳定的水溶性非分泌蛋白,含有典型的MBF1结构域和α螺旋-转角-α螺旋模序。亚细胞定位分析表明,LlMBF1a主要在细胞核中表达。LlMBF1a在根、茎、鳞茎、叶中的表达量无显著差异,受灰葡萄孢菌侵染诱导表达且在相对抗病品种中持续高水平表达,表明LlMBF1a可能参与了百合对灰霉病的抗性反应。Previous studies revealed that MBF1a was involved in host defense response.The MBF1a homologous gene was isolated from lily cultivar White Heaven by silico cloning and RT-PCR approaches.The open reading frame of LlMBF1a gene was 429 bp,encoding a protein of 142 amino acid residues,and the molecular weight of LlMBF1a protein was 15.53 ku with a theoretical isoelectric point of 10.12.Bioinformatics analysis predicted that LlMBF1a was a stable soluble non-secreted protein,containing a typical MBF1 motif and a helix-turn-helix motif.Subcellular localization assay indicated LlMBF1a expressed in nucleus.The expression of LlMBF1a was at a similar level in leaf,stem,bulb and root,and was induced by Botrytis cinerea.The up-regulation of LlMBF1a in resistant variety was higher and more persistent than that in susceptible variety,suggesting LlMBF1a might be correlated to the resistance of lily against gray mold.

关 键 词：百合 MBF1 灰葡萄孢菌 基因克隆 亚细胞定位 基因表达 

分 类 号：S682.265[农业科学—观赏园艺]