两种仪器定量检测HIV-1病毒载量比较研究  

Comparative evaluation of two detection systems for quantitative detection of HIV-1 viral load

作　　者：李丽华 余婷婷 丁彩梅 张润武 杨冬梅 王红英 白经 普冬 LI Lihua;YU Tingting;DING Caimei;ZHANG Runwu;YANG Dongmei;WANG Hongying;BAI Jing;PU Dong(Department of Laboratory Medicine,the Third People′s Hospital ofKunming,Kunming,Yunnan 650041,China)

机构地区：[1]云南省昆明市第三人民医院检验科,650041

出　　处：《检验医学与临床》2018年第24期3678-3681,共4页Laboratory Medicine and Clinic

基　　金：云南省应用基础研究青年项目(2017FD186)

摘　　要：目的比较雅培m2000和罗氏AmpliPrep/TaqMan两种全自动分析系统定量检测人类免疫缺陷病毒(HIV)-1病毒载量的一致性,为临床选择高精度检测方法提供参考。方法对30例艾滋病患者血浆标本同时采用雅培m2000全自动分析系统和罗氏AmpliPrep/TaqMan全自动分析仪进行病毒载量测定,比较二者检出率、相关性和一致性。结果雅培m2000全自动分析系统和罗氏AmpliPrep/TaqMan全自动分析仪测得HIV-1病毒载量平均值分别为(2.990±0.284)log copy/mL和(3.100±0.244)log copy/mL,差异无统计学意义(t=1.446,P>0.05);两种检测系统检测出的病毒载量有明显相关性(r=0.968,P<0.001),且与患者的CD4+T细胞数及CD4+/CD8+呈明显直线负相关。结论雅培m2000全自动分析系统和罗氏AmpliPrep/TaqMan全自动分析仪检测HIV-1病毒载量具有较好的相关性和一致性,在实际工作中,均可作为高精度检测HIV-1的可靠方法。Objective To compare the consistency of quantitative detection of HIV-1viral load between two automatic analysis systems of Abbott m2000and Roche AmpliPrep/TaqMan,and to provide reference for clinical selection of high-precision detection methods.Methods Plasma samples of30patients with acquired immune deficiency syndrome(AIDS)were measured simultaneously by Abbott m2000automatic analysis system and Roche AmpliPrep/TaqMan automatic analyzer.The detection rate,correlation and consistency between Abbott m2000Real Time System and COBAS AmpliPrep/TaqMan detection system were compared.Results The average load of HIV-1virus was(2.990±0.284)log copy/mL and(3.100±0.244)log copy/mL,respectively.There was no significant difference between the two detection systems(P>0.05).The viral load detected by the two methods was significantly correlated(r=0.968,P<0.001),and was negatively correlated with the number of CD4+T cells and CD4+/CD8+in patients.Conclusion Two systems,Abbott m2000automatic analysis system and Roche AmpliPrep/TaqMan automatic analyzer,have good correlation and consistency in detecting HIV-1virus load.Therefore,they can be used as a reliable method to detect HIV-1virus with high accuracy.

关 键 词：HIV病毒载量 雅培m2000全自动分析系统 罗氏AmpliPrep/TaqMan全自动分析仪 高精度 

分 类 号：R33[医药卫生—人体生理学]