一株DDT降解菌株D3的安全评估  

Safety Evaluation of a Strain of DDT Degrading Bacteria D3

作　　者：吴雨龙 吴向华 王俏娜 华春 WU Yu-long;WU Xiang-hua;WANG Qiao-na;HUA Chun(School of Food Science, Nanjing Xiaozhuang University, Nanjing 211171, Jiangsu;Jiangsu Provincial Key Construction Laboratory of Special Biomass Waste Resource Utilization, Nanjing 211171, Jiangsu)

机构地区：[1]南京晓庄学院食品科学学院,江苏南京211171 [2]江苏省高校"特殊生物质废弃物资源化利用"重点建设实验室,江苏南京211171

出　　处：《南京晓庄学院学报》2018年第6期68-75,共8页Journal of Nanjing Xiaozhuang University

基　　金：国家高技术研究发展计划(2012AA021701);国家自然科学基金(21376112);江苏省自然科学基金(BK20141081);江苏省高校重点建设实验室项目(苏教科[2016]8);江苏省高等学校大学生创新创业训练计划项目(201611460001Z);南京晓庄学院青年专项(2016NXY45)

摘　　要：[目的]评估DDT降解菌株D3的生物安全性.[方法]以两种毒理学方法(急性毒性试验和亚慢性毒性试验)评估DDT降解菌株D3对哺乳动物的安全性.[结果]急性毒性试验中,大鼠未出现明显中毒症状和死亡情况,且DDT降解菌株D3对大鼠的口服半数致死量(median lethal dose,LD50)超过5000 mg/kg(6. 3×1011CFU/kg);亚慢性毒性试验中,试验各组与空白对照组在相关指标对比中无明显差异.[结论]DDT降解菌株D3对哺乳动物安全无毒副作用,可以作为治理DDT污染土壤的修复剂推广应用.This study is aimed to evaluate of the biosafety of DDT degrading bacteria D3.Two toxicological methods(acute toxicity test and subchronic toxicity test)were used to evaluate the safety of DDT degrading strain D3in mammals.Results showed that there were no significant signs of toxicity and mortality in acute toxicity study,and the oral LD50of DDT degrading bacteria D3was more than5000mg/kg(6.3×10^11CFU/kg).In the subchronic toxicity test,there was no significant difference between the test groups and the blank control group in the correlation indexes.It is concluded that DDT degrading bacteria D3has no toxic and side effects to mammals and can be used as a remediation agent for DDT contaminated soil.

关 键 词：DDT降解菌株D3 安全评估 

分 类 号：Q93[生物学—微生物学]