单核苷酸多态性结合位点C19007T与宫颈癌患者易感性的相关研究  

Study on correlation between single nucleotide polymorphisms binding site C19007T and susceptibility in patients with cervical cancer

作　　者：秦梅[1] 武红权[2] 厉勇[3] 杨旭[1] 胡苑苑 QIN Mei;WU Hongquan;LI Yong;YANG Xu;HU Yuanyuan(Department of Blood Transfusion,Guiyang Municipal First People′s Hospital,Guiyang,Guizhou 550002,China;Department of Clinical Laboratory,Guizhou Provincial People′s Hospital,Guiyang,Guizhou 550002,China;Department of Blood Transfusion,Guiyang Municipal Maternal and Child Health Care Hospital,Guiyang,Guizhou 550002,China)

机构地区：[1]贵州省贵阳市第一人民医院输血科,550002 [2]贵州省人民医院检验科,贵阳550002 [3]贵州省贵阳市妇幼保健院输血科,550003

出　　处：《重庆医学》2018年第34期4395-4398,共4页Chongqing medicine

基　　金：贵州省卫生计生委科技基金项目(gzwjkj 2014-2-090)

摘　　要：目的初步探讨caspase-3基因3′末端的非翻译区microRNA的结合位点C19007T单核苷酸多态性与宫颈癌患者易感性的相关性。方法运用PCR-PIRA技术对100例宫颈癌患者和100名未检出宫颈病变妇女的C19007T多态性位点行基因分型检测。应用免疫组织化学分析癌组织中caspase-3基因的蛋白表达。结果有淋巴转移组的caspase-3C19007T的C/C、T/C、T/T基因型频率分别为31.0%(18/58)、35.1%(13/37)、20.0%(1/5),无转移组的分别为69.0%(40/58)、64.9%(24/37)、80.0%(4/5),两组基因型的分布频率差异均无统计学意义;caspase-3蛋白在宫颈鳞癌组织中的表达降低,免疫组织化学结果提示,caspase-3蛋白的表达情况与C19007T的基因型无显著相关性。结论 caspase-3基因的C19007T位点与宫颈鳞状细胞癌的发生有一定的相关性,基因型T/T可作为罹患宫颈癌风险的预测指标。Objective To preliminarily investigate the correlation between the C19007T single nucleotide polymorphism at the untranslated region microRNA of 3′end of caspase-3 gene and the susceptibility in the patients with cervical cancer.Methods The genotyping detection of C19007T polymorphism loci was performed in 100 patients with cervical cancer and 100 women without cervical lesions by PCR-PIRA.The immunohistochemical method was employed to analyze the protein expression of caspase-3 gene in cancer tissues.Results The C/C,T/C and T/T genotype frequencies in the lymphatic metastasis group were 31.0%(18/58),35.1%(13/37)and 20%(1/5)respectively,while which in the group without metastasis were 69.0%(40/58),64.9%(24/37)and 80%(4/5)respectively.There were no significant differences in the distribution frequencies of genotypes between the two groups.The expression of caspase-3 protein in cervical squamous cell carcinoma tissues was decreased.The results of immunohistochemistry showed that there was no significant correlation between caspase-3 protein expression and C19007T genotype.Conclusion The C19007T gene locus of caspase-3 gene has a certain correlation with the occurrence of cervical squamous cell carcinoma,and the genotype T/T can be used as a predictive indicator for suffering from cervical cancer risk.

关 键 词：宫颈肿瘤 CASPASE-3基因 MICRORNA 多态性 单核苷酸 

分 类 号：R711.74[医药卫生—妇产科学]