不同工艺的绞股蓝总甙的代谢组学研究  

Study on the metabonomics of gypenosides with different processes

作　　者：朱东海[1] 张偲偲 王德勤[1] 李楚源[1] 匡艳辉[1] ZHU Donghai;ZHANG Sisi;WANG Deqin;LI Chuyuan;KUANG Yanhui(Guangzhou Baiyun Mountain and Hutchison Whampoa Ltd.,Guangzhou510515,China)

机构地区：[1]广州白云山和记黄埔中药有限公司,广东广州510515

出　　处：《广东药科大学学报》2018年第6期778-783,共6页Journal of Guangdong Pharmaceutical University

基　　金：广州市科技计划项目-珠江科技新星(201710010124)

摘　　要：目的探讨不同工艺的绞股蓝总甙对高血脂大鼠血浆内源性代谢物的影响,寻找差异代谢物。方法 70只健康大鼠,随机分为正常对照组、模型组、工艺一剂量组、工艺二低、中、高剂量组和阿托伐他汀钙组。除正常对照组外,其余各组均饲喂高脂高胆固醇饲料。利用超高效液相色谱-飞行时间质朴(UPLC-Q-TOF-MS)代谢组学技术平台采集数据,所得数据采用偏最小二乘法判别分析法(PLS-DA)进行模式识别分析,筛选可能的代谢物标志物。结果不同工艺的绞股蓝总甙处理组中,只有工艺二高剂量组与模型组分别呈现聚类分布,且明显区分(P<0.05),表明与模型组相比工艺二高剂量组大鼠的血浆存在差异性的代谢物,经谱库检索和筛选分析,最终得到9个潜在生物标记物。结论基于UPLC-QTOF-MS代谢组学技术能够有效区分对照组、模型组和工艺二高剂量绞股蓝总甙组之间的代谢谱,为绞股蓝总甙治疗高血脂的作用机制提供参考。Objective To investigate the effect of gypenosides with different process on plasma endogenous metabolites in hyperlipidemia rats,and find out the different metabolites.Methods70healthy rats were randomly divided into the normal control group,the model group,process1high group,process2low,medium and high groups,and atorvastatin calcium group.Except for the control group,all the other groups were fed with high fat and high cholesterol diet.Data were collected by the super high performance liquid chromatography-mass spectrometry(UPLC-Q-TOF-MS)platform,and were analyzed by the partial least squares discriminant analysis(PLS-DA).The possible metabolites were selected.Results Among all gypenosides groups,only process2high group and the model group showed the cluster distribution(P<0.05),which suggested that there were different metabolites in the plasma of two groups.9potential biomarkers were finally determined by the spectral library retrieval and screening analysis.Conclusion Based on UPLC-Q-TOF-MS metabonomics,the metabolic spectrum of process2high group can be effectively distinguished from the control and model groups,which provides a reference for study of the mechanism of gypenosides in the treatment of hyperlipidemia.

关 键 词：绞股蓝总甙 代谢组学 高血脂 

分 类 号：Q591[生物学—生物化学]