冠心病患者CD16^+单核细胞比例与体质量指数之间的相关性研究  

Correlation between CD16^+ monocyte percentage and body mass index in coronary heart disease patients

作　　者：庞博[1] 张丹丹[2] 赵娟[1] 武婧[2] 侯雪筠[1] 叶红蕾 谷春 崔向宁[2] 刘贵建[1] PANG Bo;ZHANG Dandan;ZHAO Juan;WU Jing;HOU Xueyun;YE Honglei;GU Chun;CUI Xiangning;LIU Guijian(Department of Clinical Laboratory,Guang′anmen Hospital,China Academy of Chinese Medical Sciences,Beijing 100053,China;Department of Cardiology,Guang′anmen Hospital,China Academy of Chinese Medical Sciences,Beijing 100053,China))

机构地区：[1]中国中医科学院广安门医院检验科,北京100053 [2]中国中医科学院广安门医院心血管科,北京100053

出　　处：《国际检验医学杂志》2018年第24期3019-3022,共4页International Journal of Laboratory Medicine

基　　金：国家中医药管理局国家中医临床研究基地业务建设科研专项课题(JDZX2015247)

摘　　要：目的体质量指数(BMI)增加是较为公认的冠心病的危险因素之一,单核细胞也与冠心病的发生关系密切。本研究旨在探索冠心病患者中不同亚群单核细胞比例与BMI是否存在关联。方法连续纳入冠心病患者共48例,以BMI=25kg/m2将其分为2组,体质量正常组21例,体质量增高组27例。收集患者一般情况、血常规等临床检测指标,利用流式细胞仪检测CD14、CD16等指标,用合适的圈门策略分析不同单核细胞亚群占全部单核细胞的比例。将结果用SPSS软件进行统计分析。结果体质量增高组CD16+单核细胞比例显著高于体质量正常组[(17.2±8.1)%vs.(12.6±4.8)%,P=0.025],其余单核细胞亚群有一定变化趋势但并不显著。其他指标如白细胞总数、中性粒细胞比例、单核细胞比例等差异均无统计学意义(P>0.05)。相关性分析显示,CD16+单核细胞比例与BMI呈显著正相关(r=0.331,P=0.020)。结论在冠心病患者中,CD16+单核细胞比例与BMI之间存在显著关联性,研究BMI、炎症状态以及冠心病三者之间的关系可能为理解冠心病发生发展的分子机制提供帮助。Objective Body mass index(BMI)is one of the most acknowledged risk factors for coronary artery disease.Monocyte is also involved in the occurrence of coronary artery disease.The purpose of this study is to investigate whether the proportion of monocytes in different subsets was associated with BMI in patients with coronary artery disease.Methods A total of48consecutive patients with coronary artery disease were enrolled.They were divided into two groups according to BMI(the cutoff value was25kg/m^2).There were21patients in the normal weight group and27patients in overweighted or obese group.General information of the patients was collected,as well as the clinical test results including routine blood test results,et al.Cell surface markers of CD14and CD16were detected by flow cytometry,and the proportion of different monocyte subsets was analyzed by flow cytometry.The results were statistically analyzed by SPSS software.Results The proportion of CD16+monocytes in the overweighted group was significantly higher than that in the normal BMI group[(17.2±8.1)%vs.(12.6±4.8)%,P=0.025],and the other monocyte subsets showed a certain change trend but not significant.There was no significant difference in other parameters,such as the total number of white blood cells,the proportion of neutrophils and the proportion of monocytes(P>0.05).Correlation analysis showed that the proportion of CD16^+monocytes was positively correlated with BMI(r=0.331,P=0.020).Conclusion The difference between CD16^+monocyte percentage and BMI in patients with coronary heart disease was significant.Further study of the relationship among BMI,inflammatory state,and coronary artery disease may help to understand the molecular mechanisms of the occurrence and development of coronary artery disease.

关 键 词：冠心病 单核细胞 体质量指数 肥胖 

分 类 号：R541.4[医药卫生—心血管疾病]