探究桂枝、附子配伍含药血清的制备方案  

Preparation of Medicated Serum Containing Guizhi and Fuzi

作　　者：简旖沫 汤小虎[2] 赖张凤 JIAN Yi-mo;TANG Xiao-hu;LAI Zhang-feng

机构地区：[1]云南省中医医院,云南昆明650021 [2]云南中医学院,云南昆明650011

出　　处：《风湿病与关节炎》2018年第12期29-34,共6页Rheumatism and Arthritis

基　　金：国家自然科学基金(81060285);云南省高校科技创新团队支持计划资助(IRTSTYN);云南省应用基础研究计划项目[2015FB205(-032)]

摘　　要：目的:通过高效液相色谱法(HPLC)考察桂枝、附子配伍含药血清的制备方案。方法:将35只雄性Wistar大鼠随机分为正常组、单倍剂量组、5倍剂量组、10倍剂量组和20倍剂量组,每组7只。分别采用单倍、5倍、10倍、20倍的给药剂量予桂枝、附子配伍灌胃,正常组予生理盐水灌胃。于给药后15,30,60,90,120,180,240 min肝门静脉取血,采用甲醇沉淀法、乙腈沉淀法、三氯乙酸沉淀法以及乙酸乙酯沉淀法对血清进行处理,通过HPLC技术对血清进行检测分析。结果:采用20倍剂量给药60 min后取血,经甲醇沉淀法处理制作的含药血清可以检测到入血成分较多,色谱峰的分离效果好、离子强度高且干扰小。结论:通过HPLC法考察含药血清的制备方案可以为探讨中药配伍入血成分的物质基础提供理论依据。Objective:To investigate the preparation of medicated serum containing Guizhi(Cinnamomum cassia Presl)and Fuzi(Aconitum carmichaeli Debx)by high performance liquid chromatography(HPLC).Methods:Thirty-five male Wistar rats were randomly divided into a normal group,a one-dose group,a five-dose group,a ten-dose group and a twenty-dose group,7rats in each group.Rats in each treatment group were given intragastric administration with corresponding doses of Guizhi and Fuzi and those in the normal group were given intragastric administration with normal saline.Respectively after15,30,60,90,120,180,and240minutes of administration,blood was collected from hepatic portal vein.Serum was treated by methanol precipitation,acetonitrile precipitation,trichloroacetic acid precipitation and ethyl acetate precipitation,detected and analyzed by HPLC.Results:Medicated serum was better in the twenty-dose group with blood taken after60minutes,whose chromatographic peaks had a good separation effect,with high ionic strength and little interference.Conclusion:The preparation scheme of medicated serum by HPLC can provide theoretical basis for exploring the compatible blood components of Chinese medicine.

关 键 词：关节炎 类风湿 含药血清 桂枝 附子 HPLC 采血时间 给药剂量 

分 类 号：R289.1[医药卫生—方剂学]