‘杨氏金红50号’猕猴桃的离体快繁研究  

Micropropagation in vitro of Actinidia chinensis ‘Yangshi Jinhong 50'

作　　者：杨迪 赵新仕 邹婷婷 王赟 周业皓 杜戈 李书林 张乃群[1] YANG Di;ZHAO Xinshi;ZOU Tingting;WANG Yun;ZHOU Yehao;DU Ge;LI Shulin;ZHANG Naiqun(College of Life Sciences and Technology,Nanyang Normal University,Nanyang 473061,Henan,China;Institute of Actinidia Chinese in Xixia County,Xixia 474573,Henan,China)

机构地区：[1]南阳师范学院生命科学与技术学院,河南南阳473061 [2]西峡猕猴桃研究所,河南西峡474573

出　　处：《广西植物》2018年第12期1667-1674,共8页Guihaia

基　　金：河南省科技攻关项目(102102110159);南阳师范学院2018年度STP项目(2018STP001);南阳师范学院2017年度研究生创新基金(2017CX007)~~

摘　　要：为建立‘杨氏金红50号’猕猴桃的离体快繁体系,该研究以其带腋芽茎段为外植体,采用组织培养的方法进行离体培养,并建立了两种离体再生途径:途径I为直接诱导茎段腋芽出芽;途径Ⅱ为茎段基部先产生愈伤组织,再分化不定芽。结果表明:‘杨氏金红50号’猕猴桃带腋芽茎段的最佳灭菌方式为75%酒精30 s+15%Ca(Cl O)_25 min+0.1%升汞8 min;在途径I中,诱导茎段腋芽出芽的最优培养基为MS+4.0 mg·L^(-1)6-BA+0.1 mg·L^(-1)NAA;在途径Ⅱ中,诱导茎段基部产生愈伤组织并产生不定芽的最优培养基为MS+3.0 mg·L^(-1)6-BA+0.3 mg·L^(-1)NAA;培养丛生芽的最佳植物生长调节剂组合为MS+4.0 mg·L^(-1)6-BA+0.4 mg·L^(-1)NAA;不定芽生根培养的最佳植物生长调节剂组合为1/2 MS+0.9 mg·L^(-1)IBA,20 d左右分化出不定根,40 d左右获得完整植株;生根后的组培苗在田园土∶细沙=1∶1的基质中能达到96%的移栽成活率。In order to establish the rapid and efficient propagation system in vitro,we used stem with axillary bud of Actinidia chinensis‘Yangshi Jinhong 50’as the explant,and used tissue culture to study suitable explants sterilization method,best plant growth regulator combination in Actinidia chinensis‘Yangshi Jinhong 50’.We established two kinds of in vitro regeneration modes.Mode I:the axillary buds of stem with axillary bud were induced directly;ModeⅡ:the callus was induced first,and then the adventitious buds were induced.The results showed that the best sterilization method for stems with axillary buds was 75%alcohol 30 s+15%Ca(ClO)25 min+0.1%mercuric chloride 8 min;In Mode I,the plant growth substances combination of MS+4.0 mg·L^-1 6-BA+0.1 mg·L^-1 NAA for axillary bud germination had the best the induction rate;In ModeⅡ,callus rate of inducing stem bottom was more than 80%,the optimal medium that induced stems bottom callus to generate adventitious buds was MS+3.0 mg·L^-1 6-BA+0.3 mg·L^-1 NAA;the best plant growth substances combination in tufted bud culture was MS+4.0 mg·L^-1 6-BA+0.4 mg·L^-1 NAA;the best plant growth substances combination in rooting culture was 1/2 MS+0.9 mg·L^-1 IBA,getting the differentiation of adventitious root about 20 d and complete plant 40 d.After the seedlings rooted,transplanted the seedlings to the substrate,the seedlings reached the 96%survival rate in the substrate with the garden soil∶sand=1∶1.Through this study,the micropropagation system in vitro of Actinidia chinensis‘Yangshi Jinhong 50’was established,which provides the basis for the research of genetic transformation in Actinidia chinensis Planch.

关 键 词：'杨氏金红50号’猕猴桃 带腋芽茎段 组织培养 植物生长调节剂 再生体系 

分 类 号：Q945[生物学—植物学]