甘蓝晚抽薹基因BoFLC3克隆、序列分析和亚细胞定位  

Cloning,sequence analysis and subcellular localization of Brassica oleraceaBoFLC3 gene

作　　者：戴忠良[1] 陈丽 山溪 秦文斌[1] 肖燕[1] 朱建飞[1] DAI Zhong-liang;CHEN Li;SHAN Xi;QIN Wen-bin;XIAO Yan;ZHU Jian-fei(Zhenjiang Institute of Agricultural Sciences in the Ning-Zhen Hilly District,Jurong 212400,China;Department of Horiticulture,Nanjing Agricultural University,Nanjing 210095,China)

机构地区：[1]江苏丘陵地区镇江农业科学研究所,江苏句容212400 [2]南京农业大学园艺学院,江苏南京210095

出　　处：《江苏农业学报》2018年第6期1324-1330,共7页Jiangsu Journal of Agricultural Sciences

基　　金：江苏省农业科技自主创新基金项目[CX(18)2006]

摘　　要：参照甘蓝FLC3基因(GenBank登录号AAP31677)碱基序列设计引物,扩增出甘蓝晚抽薹BoFLC3基因,序列分析结果表明,该基因编码的蛋白质有197个氨基酸,分子量20 990,等电点9. 45。荧光定量PCR分析结果表明:抽薹后BoFLC3基因在甘蓝不同部位表达存在差异,表达量从高到低依次为:叶、茎、花蕾、花瓣和根。亚细胞定位结果表明:BoFLC3蛋白定位在细胞核。According to the sequence of FLC3 gene(GenBank accession number AAP31677),primers were designed to amplify the BoFLC3 gene of Brassica oleracea L.Sequence analysis showed that the gene encoded a protein containing 197 amino acids,the molecular was 20 990,and the isoelectric point was 9.45.Quantitative real-time PCR analysis results showed that the expression of BoFLC3 gene in different parts of cabbage after bolting was different,and the order of expression from high to low was leaves,stems,buds,flowers and roots.Subcellular localization results showed that the BoFLC3 protein was located in the nucleus.

关 键 词：甘蓝 BoFLC3基因 荧光定量PCR 亚细胞定位 序列分析 

分 类 号：Q785[生物学—分子生物学]