亚麻芥未成熟胚中磷脂酶基因家族的转录组学分析  

Transcriptome analysis of phospholipase gene family in immature embryo of Camelina sativa

作　　者：李晓薇 戢舒涵 赵旭 张百兵 王法微 李海燕 LI Xiao-wei;JI Shu-han;ZHAO Xu;ZHANG Bai-bing;WANG Fa-wei;LI Hai-yan(College of Life Sciences,Jilin Agricultural University/Engineering Research Center of Bioreactor and Pharmaceutical Development,Ministry of Education,Changchun130118,China;Jilin Anxin Food Technology Services Limited Company,Changchun130033,China)

机构地区：[1]吉林农业大学生命科学学院/生物反应器与药物开发教育部工程研究中心,吉林长春130118 [2]吉林省安信食品技术服务有限责任公司,吉林长春130033

出　　处：《中国油料作物学报》2018年第6期793-800,共8页Chinese Journal of Oil Crop Sciences

基　　金：国家自然科学基金项目(31601323;31201144);吉林省科技厅项目(20170101015JC);国家级大学生创新创业训练计划项目(201610193043)

摘　　要：为了解磷脂酶基因家族在亚麻芥胚中的表达情况,本研究基于454测序技术对亚麻芥花后10d(DAF10)和花后20d(DAF20)的胚中磷脂酶基因家族进行筛选和分析。测序结果表明,DAF10样本和DAF20样本分别获得有效序列521 507个和310 125个,序列长度主要分布在341～560 bp,序列组装分别获得25 398个和23 678个Unigene,Unigene平均长度分别为630 bp和654 bp。在此基础上,筛选两个样本全部Unigene,共获得磷脂酶基因36个,其中属于磷脂酶A1基因4个、磷脂酶A2基因9个、磷脂酶C基因10个、磷脂酶D基因13个。Pathway基因功能注释显示,PLA1的4个候选基因没有注释到任何代谢通路上;PLA2中只有候选基因Unigene19110被成功注释,它在植物中主要参与甘油磷脂代谢途径和醚酯代谢途径。PLC家族中只有4个候选成员Unigene443、Unigene8071、Unigene8213和Unigene7311被成功注释,它们除参与甘油磷脂代谢和醚酯代谢之外,还在肌醇磷酸代谢途径中发挥重要作用。而PLD家族中除Unigene18630、Unigene17966和Unigene441没有代谢通路注释信息外,其他的10个成员,也都只在甘油磷脂代谢途径和醚酯代谢途径有作用。In order to obtain information of phospholipases in Camelina sativa,transcriptome profiles of embryos at two developmental stages of 10 days( DAF10) and 20 days( DAF20) after flowering were analyzed by 454 pyrosequencing. A total of 521 507 and 310 125 clean reads were produced in DAF10 and DAF20,respectively.The distribution of clean reads length was mainly 341 bp-560 bp. 25 398 and 23 678 unigenes were obtained with the average length of 630 bp and 654 bp in DAF10 and DAF20 by de novo assembly,respectively. On this basis,we screened all unigenes of these two samples and obtained 36 candidate phospholipase genes. 36 phospholipase genes were divided into 4 classes,and 4 were phospholipase A1,9 were phospholipase A2,10 were phospholipase C,and 13 were phospholipase D. The phospholipase gene family mainly involved in glycerophospholipid metabolism,ether lipid metabolism and inositol phosphate metabolism pathway,as shown in Pathway analysis. In details,all 4 PLA1 genes did not participate in any metabolism pathway,and only Unigene19110,one of PLA2 nine members,played an important function in glycerophospholipid metabolism and ether lipid metabolism. PLC genes,Unigene443,Unigene8071,Unigene8213 and Unigene7311,not only took part in glycerophospholipid metabolism and ether lipid metabolism,but also in inositol phosphate metabolism. Among 13 PLD members,10 of them except Unigene18630,Unigene17966 and Unigene441 were all involved in glycerophospholipid metabolism and ether lipid metabolism pathway.

关 键 词：亚麻芥 转录组 磷脂酶 基因 代谢通路 

分 类 号：S565.9[农业科学—作物学]