不同人群结核分枝杆菌检测方法的比较  

Comparison of detection methods of mycobacterium tuberculosis in different populations

作　　者：赵芳 李超 赵缜 ZHAO Fang;LI Chao;ZHAO Zhen(Department of Clinical laboratory,Shanghai Minhang District Central Hospital,Shanghai 201199,China)

机构地区：[1]上海市闵行区中心医院,上海201119

出　　处：《国际检验医学杂志》2018年第A02期1-3,共3页International Journal of Laboratory Medicine

基　　金：国家高科技研究发展计划子课题(2015AA021107-035)。

摘　　要：目的探讨抗酸杆菌涂片染色法、MGIT液体培养法在不同人群中检测效果和应用价值。方法选取2017年1~4月在上海市闵行区中心医院结核门诊的初诊患者158例和随访患者165例。每个患者分别采集痰液标本4份,分别采用抗酸杆菌涂片染色法、分枝杆菌MGIT液体培养法检测结核分枝杆菌,并对其检出率进行比较。结果158例初诊患者中,痰涂片抗酸杆菌阳性率为12.0%(19/158),MGIT液体培养法阳性率为20.9%(33/158),两组差异具有统计学意义(P<0.05);165例随访患者中,痰涂片阳性率为3.6%(6/165),MGIT液体培养法阳性率为4.2%(7/165),两组差异无统计学意义(P>0.05)。初诊患者TB阳性率为20.9%,显著高于随访患者的TB阳性率(4.2%)。结论为了提高结核的确诊率,建议对初诊患者采用2种方法联合检侧。Objective To investigate the detection effect and application value of sputum spear acid-fast stain method and Mycobacterium MGIT liquid culture method in different populations.Methods 158 first visit patients and 165 follow-up patients were selected in tuberculosis outpatient department of Shanghai Minhang District Hospital from January 2017 to April 2017.Four sputum specimens were collected from each patient,and detected using sputum spear acid-fast stain method and Mycobacterium MGIT liquid culture method,respectively.Results The positive rate of tuberculosis was 12.0%(19/158)using sputum spear acid-fast stain method and 20.9%(33/158)using Mycobacterium MGIT liquid culture method in 158 first visit patients.The difference was statistically significant(P<0.05).The positive rate of tuberculosis was 3.6%(6/165)by sputum spear acid-fast stain method and 4.2%(7/165)by Mycobacterium MGIT liquid culture method in 165 follow-up patients.The difference was no statistically significant(P>0.05).The positive rate of tuberculosis in first visit patients was 20.9%,which was significantly higher than that(4.2%)in follow-up patients.Conclusion In order to improve the diagnosis rate of TB patients,it is suggested that two methods should be combined to examine the first visit patients in tuberculosis outpatient department.

关 键 词：结核分枝杆菌 抗酸杆菌涂片染色法 MGIT液体培养法 

分 类 号：R378.911[医药卫生—病原生物学]