儿童过敏性紫癜血清中IFN-γ、IL-10、TGF-β、PFP、GNLY的表达及意义  

Expressions of IFN-γ,IL-10,TGF-β,PFP and GNLY as well as their significances in serum of children with Henoch Schonlein purpura

作　　者：白涛敏[1] BAI Tao-min(Department of Pediatrics,Shaanxi People’s Hospital,Xi’an,Shaanxi,710068,China)

机构地区：[1]陕西省人民医院儿科,陕西西安710068

出　　处：《海南医学院学报》2018年第23期2054-2057,共4页Journal of Hainan Medical University

基　　金：陕西省社会发展项目(2017SF-239)~~

摘　　要：目的:分析过敏性紫癜(HSP)患儿血清中干扰素-γ(IFN-γ)、白细胞介素10(IL-10)和转化生长因子-β(TGF-β)及穿孔素(PFP)、颗粒溶素(GNLY)的表达水平,并探讨其表达意义。方法:选择本院收治的86例HSP患儿作为研究对象(HSP组),根据是否合并肾损伤分为肾损伤组41例和无肾损伤组45例,同时期40例健康儿童为对照组。采用酶联免疫吸附实验(ELISA)检测受试儿童血清IFN-γ、IL-10和TGF-β表达水平,RT-PCR检测血清PFP、GNLY表达水平,比较不同组之间差异,并分析HSP组血清IFN-γ、IL-10、TGF-β、PFP、GNLY之间的关系。结果:HSP组血清IFN-γ、PFP、GNLY水平低于对照组(P<0.05),血清IL-10、TGF-β水平高于对照组(P<0.05);肾损害组血清IFN-γ、PFP、GNLY水平低于无肾损害组(P<0.05),血清IL-10、TGF-β水平高于无肾损害组(P<0.05);HSP组血清IFN-γ与TGF-β呈负相关关系(r=-0.417,P=0.037),与PFP、GNLY分别呈正相关关系(r=0.508、0.477,P=0.025、0.030);IL-10与TGF-β呈正相关关系(r=0.514,P=0.017)。结论:血清IFN-γ、IL-10、TGF-β、PFP、GNLY水平变化与HSP发生及进展密切相关,早期检测有助于HSP病情评估,对临床预防过敏性紫癜肾炎的发生具有指导意义。Objective:To investigate the expressions of interferon-γ(IFN-γ),interleukin-10(IL-10),transforming growth factor-β(TGF-β),perforin(PFP)and granulysin(GNLY)in serum of children with Henoch-Schonlein purpura(HSP)and their significances.Methods:A total of 86 children with HSP admitted to our hospital from April 2016 to April 2018 were selected as the study subjects(HSP group).According to whether the disease was combined with renal injury or not,the children were divided into kidney injury group(41 cases)and non-kidney injury group(45 cases).Besides,40 healthy children were selected as the control group.The levels of serum IFN-γ,IL-10 and TGF-βwere detected by enzyme-linked immunosorbent assay(ELISA),while levels of serum PFP and GNLY were detected by RT-PCR.The differences between different groups were compared,and the relationship of serum IFN-γ,IL-10,TGF-β,PFP and GNLY in HSP group was also analyzed.Results:The levels of serum IFN-γ,PFP and GNLY in HSP group were lower than those in control group(P<0.05),while the levels of serum IL-10 and TGF-βwere higher than those in control group(P<0.05);the levels of serum IFN-γ,PFP and GNLY in renal injury group were lower than those in non-renal injury group(P<0.05),while the levels of serum IL-10 and TGF-βwere higher than those in non-renal injury group(P<0.05);in HSP group,serum IFN-γwas negatively correlated with TGF-β(r=-0.417,P=0.037),and positively correlated with PFP and GNLY(r=0.508,0.477,P=0.025,0.030);there was a positive correlation between IL-10 and TGF-β(r=0.514,P=0.017).Conclusion:The changes on the levels of serum IFN-γ,IL-10,TGF-β,PFP and GNLY are closely related to the occurrence and progression of HSP,early detection is helpful to the evaluation of HSP and has guiding significance for clinical prevention of Henoch-Schonlein purpura nephritis.

关 键 词：过敏性紫癜 干扰素-Γ 白细胞介素10 转化生长因子-β 穿孔素 颗粒溶素 

分 类 号：R725.5[医药卫生—儿科]