反相高效液相色谱法测定生物质水解液中的甲酸含量  

Determination of formic acid content in hydrolysate of biomass by reversed-phase HPLC

作　　者：张萍[1] 陈燕[1] 唐斌 ZHANG Ping;CHEN Yan;TANG Bin(College of Chemistry and Chemical Engineering,Xianyang Normal University,Xianyang 712000,China)

机构地区：[1]咸阳师范学院化学与化工学院,陕西咸阳712000

出　　处：《化工科技》2018年第6期51-54,共4页Science & Technology in Chemical Industry

基　　金：国家自然科学基金项目(21305117);陕西省自然科学基金项目(2017JQ2043);陕西省教育厅专项研究基金项目(17JK0829)

摘　　要：为了给生物质水解液研究中的甲酸含量检测提供可靠的方法,建立了反相高效液相色谱法(RP-HPLC)分析生物质水解液中甲酸含量的方法。运用Ultimate LP-C18色谱柱(250mm×4.6mm,5μm),以V(NaH_2PO_4水溶液)∶V(甲醇)=97∶3[c(NaH_2PO_4)=0.02mol/L(pH=2.5)]体系为流动相,在流速1.0mL/min、柱温30℃、检测波长210nm条件下,实现了生物质水解液中甲酸的分离分析。方法评价结果表明该法测定甲酸的线性范围为0.01～0.10mol/L(相关系数R^2=0.998 7),甲酸平均回收率为96.6%～105.0%,RSD值为1.4%～2.5%(n=3)。该法测定生物质水解液中的甲酸具有操作简单易行、分析结果准确可靠的优点,可作为生物质水解液中甲酸含量分析的常规方法。A method was developed for the determination of formic acid in biomass hydrolysate by Reversed-phase high performance liquid chromatography(RP-HPLC).Ultimate LP-C18(250 mm×4.6 mm,5μm)was used as the analytical column.The mobile phase was methanol-0.02 mol/L sodium dihydrogen phosphate(V/V=97∶3),pH=2.5 and the column temperature is 30 degrees.The flow rate was 1.0 mL/min,and detection wavelength was 210 nm.The results show that the linear range of the method was 0.01~0.10 mol/L with good linear relationship(R 2=0.998 7).The average recovery was 96.6%~105.0%.The date for formic acid determined is repeatable with RSD of 1.4%~2.5%(n=3).The method is simple,realizable,accurate and reliable and can be used as a conventional method for determination of formic acid in biomass hydrolysate.

关 键 词：反相高效液相色谱 甲酸 生物质 

分 类 号：O657.7[理学—分析化学]