脑多头蚴重组蛋白rTm16和rTm-GST的免疫保护效果分析  

Immune Protective Effect Analysis of Recombinant Protein rTm16 and rTm-GST from Coenurus cerebralis

作　　者：郭承[1] 万洁[2] 杨应东[2] 文建国[2] 刘俞辰 古小彬[1] 谢跃[1] 杨光友[1] GUO Cheng;WAN Jie;YANG Ying-dong;WEN Jian-guo;LIU Yu-chen;GU Xiao-bin;XIE Yue;YANG Guang-you(College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China;Panzhihua Academy of Agricultural and Forestry Sciences, Panzhihua 617061, China)

机构地区：[1]四川农业大学动物医学院,成都611130 [2]攀枝花市农林科学研究院,攀枝花617061

出　　处：《畜牧兽医学报》2018年第12期2707-2714,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：四川省科技厅项目(2015NZ0041)

摘　　要：为探究多头带绦虫16 k Da蛋白和GST蛋白对山羊脑多头蚴病的免疫保护效果,44只攀枝花本地黑山羊随机分为4组(rTm16疫苗组、rTm-GST疫苗组、rTm16+rTm-GST疫苗组以及对照组),皮下免疫三次,首免后4周进行2免,2免后6个月进行3免,定时采血并分离血清,使用间接ELISA方法检测血清中特异性Ig G水平;末次免疫后2周,各组山羊每只口服攻击多头带绦虫的虫卵约5 500个,感染105 d后剖检记录脑多头蚴包囊数量,计算减囊率。结果显示:相较于对照组,rTm16组、rTm-GST组、rTm16+rTm-GST组分别获得50%、62. 5%、87. 5%的减囊率,rTm16+rTm-GST免疫组山羊保护效果最好(P=0. 005)。ELISA检测结果显示,攻虫以前,rTm-GST组特异性抗体水平均显著低于rTm16组(P <0. 05),而联合免疫组中rTm-GST所诱导的特异性抗体与rTm16所诱导的特异性抗体水平差异不显著(P>0. 05)。rTm16与rTm-GST的配合使用能够诱导山羊产生较高抗体水平,从而获得较显著的免疫保护力。To explore the immune protective effect of recombinant Tm16and GST proteins against Coenurus cerebralis in goats,forty-four goats were divided into four groups,including rTm16group,rTm-GST group,rTm16+rTm-GST group and control group,which were immunized3times by subcutaneous injection,respectively.Second vaccination was performed4weeks after the first vaccination,and6months later the last vaccination was performed.The serum was regularly collected from each goat,and the specific IgG response to each group was measured by indirect ELISA.Two weeks after the final vaccination,each goat was orally challenged with5500eggs of Taenia multiceps.One hundred and five days post infection all goats were slaughtered,the cysts in brain and muscle were collected and counted.Result were as follows:Compared with the control group,the cysts of goat in group rTm16,rTm-GST and rTm16+rTm-GST reduced by50%,62.5%and87.5%,respectively.Combined immunization of rTm16with rTm-GST induced significant protection(P<0.05)against challenge of T.multiceps in goats.Result of iELISA showed that the specific IgG response against rTm-GST was significantly weaker than that against rTm16(P<0.05).However,the specific IgG response against rTm-GST was not significant compared with that against rTm16in combined group(P>0.05).This revealed that the combined use of rTm16and rTm-GST could induce higher antibody levels in goats,thus obtaining significant protective immunity.

关 键 词：脑多头蚴 免疫评价 Tm16 GST 山羊 

分 类 号：S852.734[农业科学—基础兽医学]