SPE前处理-UHPLC-MS/MS测定MBR中两种N-酰基高丝氨酸内酯  

Quantitative Determination of two N-acylhomoserine Lactones in MBR by SPE Pre-processing-UHPLC-MS/MS

作　　者：黄瑾辉[1,2] 胡熠 顾岩岭 史亚慧 HUANG Jinhui;HU Yi;GU Yanling;SHI Yahui(College of Environmental Science and Engineering,Hunan University,Changsha410082,China;Key Laboratory of Environmental Biology and Pollution Control(Hunan University),Ministry of Education,Changsha410082,China)

机构地区：[1]湖南大学环境科学与工程学院,湖南长沙410082 [2]环境生物与污染控制教育部重点实验室(湖南大学),湖南长沙410082

出　　处：《湖南大学学报（自然科学版）》2018年第12期144-148,共5页Journal of Hunan University:Natural Sciences

基　　金：国家自然科学基金资助项目(51578222);教育部科学研究项目(113049A)~~

摘　　要：选用N-己基高丝氨酸内酯(C6-HSL)和N-辛基高丝氨酸内酯(C8-HSL)两种酰基高丝氨酸内酯类(AHLs)为代表物,建立了固相萃取(SPE)-超高效液相色谱串联三重四级杆质谱(UHPLC-MS/MS)分析膜生物反应器(MBR)活性污泥中AHLs的方法.研究结果表明C6-HSL和C8-HSL在1～200μg/L内有良好的线性关系(R2> 0.998),方法的检出限为0.100μg/L,定量限为1.000μg/L,在3个浓度水平下的平均加标回收率为80.69%～83.75%,相对标准偏差(RSD)为4.71%～7.25%.方法精确度高、重复性好、基质效应弱,适用于MBR活性污泥中痕量AHLs的测定.A method was developed for the determination of AHLs(N-Hexanoyl-DL-homoserine lactone(C6-HSL)and N-Octanoyl-DL-homoserine lactone(C8-HSL)were chosen as the representatives)in activated sludge in MBRs by solid phase extraction(SPE)combined with ultrahigh pressure liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS).The results showed that C6-HSL and C8-HSL presented good linear relations(R^2>0.998)in the range of1~200μg/L.The limit of detection and quantitation were0.100μg/L and1.000μg/L,respectively.The average recoveries of AHLs at three spiked concentrations were in the range of80.69%~83.75%,and the relative standard deviations(RSD)were4.71%~7.25%.The developed method is sensitive,stable and has weak matrix effects,which is applicable to determine the trace AHLs in activated sludge in MBRs.

关 键 词：膜生物反应器 N-酰基高丝氨酸内酯 固相萃取 超高效液相色谱-串联质谱 

分 类 号：X703[环境科学与工程—环境工程]