辣椒CMS系及其保持系叶绿体超微结构观察比较  

Observation and Comparison of Chloroplast Microstructure in Cytoplasmic Male-sterile line and Maintainer Line of Chili Pepper

作　　者：张茹[1,2] 陈灵芝 魏兵强[1,2] 王兰兰[1,2] ZHANG Ru;CHEN Lingzhi;WEI Bingqiang;WANG Lanlan(Institute of Vegetable,Gansu Academy of Agricultural Sciences,Lanzhou Gansu 730070,China;Northwestern Regional Vegetable Science Observation Experimental Station of Horticultural Crops biology and Germplasm Creation Group of the Ministry of Agriculture,Lanzhou Gansu 730070,China)

机构地区：[1]甘肃省农业科学院蔬菜研究所,甘肃兰州730070 [2]农业部园艺作物生物学与种质创制学科群西北地区蔬菜科学观测实验站,甘肃兰州730070

出　　处：《甘肃农业科技》2018年第12期13-16,共4页Gansu Agricultural Science and Technology

基　　金："十三五"国家重点研发计划项目(2017YFD0101903);甘肃省自然基金项目(1606RJZA122);甘肃省农业科学院博士基金项目(2016GAAS28);甘肃省科技重大专项计划(17ZD2NA015)资助

摘　　要：从辣椒胞质雄性不育系8A、保持系8B为材料,利用透射电镜观察两者叶片叶绿体超微结构,探讨CMS与叶绿体结构的关系,并比较其特性与差异,揭示CMS机理。结果表明,辣椒CMS系8A叶绿体结构发生变化,表现为基粒片层之间界限模糊、消失,发育滞后,与基粒连接的类囊体不发达,整个片层排列紊乱,叶绿体数目减少;保持系8B叶绿体形状为椭圆形,基粒片层、类囊体均发育正常。辣椒胞质雄性不育系叶绿体数目较保持系减少,结构、形状与保持系有所不同。The experiment was conducted with pepper CMS line 8A and its maintainer 8B as the chloroplast donors,the ultrastructure of chloroplast of both leaves was observed by transmission electron microscopy.The aim was to discuss the relationship between CMS and chloroplast microstructure and to reveal the mechanism of CMS by investigating chloroplast micro-structural characters in CMS male-sterile line and maintainer line.The results showed that the chloroplast structure of CMS line 8A of pepper was deformed,as the boundaries between the basal lamella were blurred and disappeared,the development was delayed,the thylakoid connecting with the grana was underdeveloped,the whole lamella arrangement was disordered,and the number of chloroplasts decreased.The shape of maintainer line 8B chloroplast was elliptic,and the basal lamella and thylakoid were all developed normally.The number of chloroplasts in the cytoplasmic male sterile lines of capsicum was less than that of the maintainer lines,and its structure and shape were different from maintainer lines.

关 键 词：辣椒 胞质雄性不育 不育系 保持系 叶绿体 超微结构 

分 类 号：S641.3[农业科学—蔬菜学]