北细辛不同药用部位黄樟醚含量测定  

Determination of Safrole in Different Parts of Asarumheterotropoides by HPLC

作　　者：姚诗琪 韩林涛[1] 黄芳[1] 汪琼[1] 李亚敏 周祯祥[1] 戴王强 Yao Shiqi;Han Lintao;Huang Fang;Wang Qiong;Li Yamin;Zhou Zhenxiang;Dai Wangqiang(Hubei University of Traditional Chinese Medicine, Wuhan 430065, China)

机构地区：[1]湖北中医药大学,湖北武汉430065

出　　处：《广东化工》2018年第23期18-19,共2页Guangdong Chemical Industry

基　　金：细辛毒性机制的代谢组学与毒理学基因组学分析方法;项目号:81573625

摘　　要：目的测定北细辛不同部位黄樟醚含量方法:采用ZORBAXSB-C18(4.6mm×150mm,5μm)色谱柱,流动相为甲醇-水,流速1.0mL·min-1,检测波长287 nm,柱温35℃。结果黄樟醚进样量在5～500μg·mL-1内线性关系良好(r=0.99970)。平均回收率为99.58%,RSD值为1.304%。结论该方法简便、可行,重现性好,为细辛不同药用部位质量控制提供参考。Objective To determinesafrolein different parts of Asarumheterotropoides Fr.Schmidt var.mandshuricum(Maxim.)Kitag.Methods ZORBAX SB-C18(4.6 mm×150 mm,5 um)as the column,the mobile phase consisted of methanol-waterat the flow rate of 1.0 mL·min^-1,the detective wavelength was 287 nm,and the column temperature was 35℃.Results safrole showed good linearity(r=0.99970)in the ranges of 50~5000μg·mL-1.The average recovery rates were 99.58%with RSD 1.304%.Conclusion This method is simple,feasible and reproducible,and provides a reference for the quality control of different parts of Asarum.

关 键 词：北细辛 黄樟醚 高效液相色谱法 含量测定 

分 类 号：TQ[化学工程]