痹通冲剂的质量标准研究  

Researching of quality standard of BiT ong granules

作　　者：李欢 石贵荣[1] 许爱珍[1] 张宏[1] 邬璟 张悦 刘美虹[1] 安明 郭晓文 LI Huan;SHI Guirong;XU Aizhen;ZHANG Hong;WU Jing;ZHANG Yue;LIU Meihong;AN Ming;GUO Xiaowen(Baotou Central Hospital,Baotou 014040,China;Baotou Medical College)

机构地区：[1]包头市中心医院制剂科,内蒙古包头014040 [2]内蒙古科技大学包头医学院

出　　处：《包头医学院学报》2018年第12期109-112,共4页Journal of Baotou Medical College

摘　　要：目的:建立中药制剂痹通冲剂的质量标准。方法:采用薄层色谱法(TLC),对痹通冲剂中的葛根和牛蒡子进行定性鉴别;采用高效液相色谱法(HPLC),对痹通冲剂中葛根素进行定量分析。色谱柱采用Agilent C18色谱柱(4. 6×250 mm,5μm),以甲醇-0. 5%冰醋酸水(23∶77)为流动相;流速1. 0 m L/min,柱温30℃,检测波长250 nm。结果:痹通冲剂中葛根素和牛蒡苷的TLC鉴别特征明显,专属性强;HPLC含量测定法线性范围为5. 00～100. 00μg/m L,浓度范围内与峰面积呈良好的线性关系(R2=0. 9999);平均加样回收率(n=9)为102. 5%(RSD=1. 63%)。结论:该实验建立的痹通冲剂中葛根素和牛蒡苷的定性鉴别与含量测定的方法简便稳定,适用于痹通冲剂的质量控制。Objective:To develop a method for Quality standard of BiTong Granules.Methods:Pueraria lobata and burdock in BiTong Granules was identified by TLC and puerarin's content was determined by HPLC.The measurement conditions were:Agilent C18Column(4.6×250mm,5μm);the methanol-0.5%glacial acetic acid(23:77)used as mobile phase;flow rate of1.0mL/min,column temperature of30℃and detection wavelength of250nm.Results:The linear range of puerarin was5.00~100.00μg/mL;there was good linear relation between concentration range and peak surface(R^2=0.9999);the average recovery(n=9)was102.5%(RSD=1.63%).Conclusion:The established methods are simple and accurate,and can be used for the quality control of BiTong Granules.

关 键 词：痹通冲剂 葛根素 牛蒡苷 鉴别 含量测定 

分 类 号：R286.0[医药卫生—中药学]