炎症条件下Ltbp2促进淋巴细胞跨内皮细胞迁移  

Ltbp2 Promotes Lymphocytes Migration of Endothelial Cells Under Inflammatory Conditions

作　　者：骈亚亚 聂晶晶 高振祥[1] 胡继红[1] PIAN Ya-Ya;NIE Jing-Jing;GAO Zhen-Xiang;HU Ji-Hong(National Center for Clinical Laboratories,Beijing Hospital,National Center of Gerontology,Beijing 100730,China)

机构地区：[1]北京医院国家老年医学中心卫生部临床检验中心,北京100730

出　　处：《生物技术通讯》2018年第6期736-742,共7页Letters in Biotechnology

基　　金：北京医院博士启动基金项目(bj-2018-027)

摘　　要：目的:探讨组织内转化生长因子β结合蛋白2(Ltbp2)在炎症条件下促进淋巴细胞及其亚群跨内皮细胞的迁移。方法:利用CRISPR/Cas9敲除MS1细胞上的Ltbp2基因;淋巴细胞与内皮细胞黏附实验比较淋巴细胞对MS1细胞和敲除细胞Ltbp2^(-/-)MS1的黏附能力,RT-PCR及流式细胞术比较MS1细胞和敲除细胞Ltbp2^(-/-)MS1表面黏附分子的表达情况;Transwell实验比较淋巴细胞及其亚群穿过MS1细胞和敲除细胞Ltbp2^(-/-)MS1的能力。结果:在炎症条件下,淋巴细胞的黏附及跨MS1细胞的迁移能力显著高于敲除细胞Ltbp2^(-/-)MS1。结论:在炎症条件下,Ltbp2促进淋巴细胞与内皮细胞的黏附及跨内皮细胞迁移。Objective:To study the latent-transforming growth factorβ-binding protein2(Ltbp2)promotes the migration of lymphocytes and their subsets through endothelial cells in inflammatory conditions.Methods:We knocked out the Ltbp2gene in MS1cells by CRISPR/Cas9technology to obtain knockout cells Ltbp2^-/-MS1.The adhesion assay for lymphocytes and endothelial cells was applied to compare the adhesion ability of MS1cells and knockout cells Ltbp2^-/-MS1.We tested the expression of adhesion molecules on MS1cells and knockout cells Ltbp2^-/-MS1by RT-PCR and flow cytometry methods.Transwell model was used to compare lymphocytes and their subsets transmigration ability of MS1cells and knockout cells Ltbp2^-/-MS1.Results:Under inflammatory conditions,the adhesion of lymphocytes to MS1cells and the ability of lymphocytes migration of MS1cells were significantly higher than those of knockout cells Ltbp2^-/-MS1.Conclusion:Under inflammatory conditions,the Ltbp2promotes the adhesion of lymphocytes to endothelial cells and migration of endothelial cells.

关 键 词：组织内转化生长因子β结合蛋白2 淋巴细胞 内皮细胞 迁移 炎症 CRISPR/Cas9 

分 类 号：Q24[生物学—细胞生物学] R392.1[医药卫生—免疫学]