基于高通量测序的生物炭施用量对植烟土壤细菌群落的影响  

Effects of Application Amount of Biochar on Soil Bacterial Community in Tobacco Fields Based on High-throughput Sequencing

作　　者：任天宝[1] 杨艳东 高卫锴[2] 李宙文 阎海涛[1] 王省伟[3] 刘国顺[1] REN Tianbao;YANG Yandong;GAO Weikai;LI Zhouwen;YAN Haita;WANG Shengwei;LIU Guoshun(Henan Agricultural University/Henan Engineering Research Center for Biochar,Zhengzhou450002,China;China Tobacco Guangdong Industrial Co.,Ltd.,Guangzhou510000,China;Zhengzhou City Tobacco Company of Henan Province,Zhengzhou450000,China)

机构地区：[1]河南农业大学/河南省生物炭工程技术中心,河南郑州450002 [2]广东中烟工业有限责任公司,广东广州510000 [3]河南省烟草公司郑州市公司,河南郑州450000

出　　处：《河南农业科学》2018年第12期64-69,共6页Journal of Henan Agricultural Sciences

基　　金：国家重点研发计划项目(2017YFD0200808);基于"双喜"品牌原料需求的湘西山地特色优质烟叶开发关键技术研究[粤烟15XM-QK(2013)-01];登封地区植烟土壤生态保育及修复技术研究及利用(ZYKJ201501)

摘　　要：以烤烟K326根系土壤为研究对象,对原始土壤(OEE)、不施用生物炭(CK)以及3个生物炭施用量递增处理(0. 75、1. 5、2. 25 t/hm^2)的根系土壤进行高通量测序,分析细菌群落多样性及组成,以期为生物炭改良山地土壤提供参考。结果表明,与CK相比,生物炭处理细菌Chao1、Shannon指数均有所降低,但生物炭的施入对放线菌门(Proteobacteria)、绿弯菌门(Chloroflexi)、装甲菌门(Armatimonadetes)的群落丰度有明显的提高作用,而对酸杆菌门(Acidobacteria)的群落丰度则表现出抑制作用,对螺旋体菌门(Saccharibacteria)、芽单胞菌门(Gemmatimonadetes)、变形菌门(Actinobacteria)群落丰度的促进或抑制作用因施用量不同而不同。生物炭处理之间细菌优势菌门组成相似度较高,与OEE细菌优势菌门组成相似度较低,与CK细菌优势菌门组成相似度较OEE高,但较其他生物炭处理低。High-throughput sequencing of root zoon soil of tobacco K326of treatments with original soil(OEE),no biochar and biochar of0.75,1.5,2.25t/ha were done,and the diversity and composition of bacteria were analyzed,so as to provide reference for improvement of mountain soils by biochar.The results showed that,compared with CK,Chao1,Shannon indices of treatments with biochar decreased,the community abundances of Proteobacteria,Chloroflexi,Armatimonadetes increased,the community abundance of Acidobacteria decreased,the community abundance of Saccharibacteria,Gemmatimonadetes and Actinobacteria changed with the application rates of biochar.There was higher similarity for dominant phyla composition among treatments with biochar,but lower between OEE and treatments with biochar,the similarity for dominant phyla composition between CK and treatments with biochar was higher than that between OEE and treatments with biochar.

关 键 词：生物炭 植烟土壤 微生物 多样性 高通量测序 

分 类 号：S572[农业科学—烟草工业]