活性黑染料脱色菌的分离鉴定及复合菌群培养研究  

Isolation and identification of reactive black dye decoloring bacterial strain and cultivating of multiple microorganisms

作　　者：杨财容 杨继鸿 祁伟亮 王芳 张硕 刘松青 YANG Cairong;YANG Jihong;QI Weiliang;WANG Fang;ZHANG Shuo;LIU Songqing(College of Chemistry and Life Science,Chengdu Normal University,Chengdu 611130,China;School of Life Sciences,Lanzhou University,Lanzhou 730000,China)

机构地区：[1]成都师范学院化学与生命科学学院,四川成都611130 [2]兰州大学生命科学学院,甘肃兰州730000

出　　处：《湖北大学学报（自然科学版）》2019年第1期26-31,38,共7页Journal of Hubei University：Natural Science

基　　金：四川省教育厅自然科学重点项目(15ZA0333)资助

摘　　要：从活性污泥中分离得到6株活性黑脱色菌,从中筛选出脱色能力最强的菌株Y1,经菌落形态、生理生化特性及16S r RNA基因序列分析,鉴定该菌株属于希瓦氏菌属(Shewanella).通过两两复配、三三复配实验探究复合菌群的脱色效果,结果表明,混合培养后复合菌群对活性黑染料的脱色率较单一菌株培养时均有较明显的提高.部分复合菌群的脱色率在72 h后稳定在90%以上,其中复合菌株Y1+Y5的脱色率最高为95. 68%,并且其对印染工业废水也具有较强的脱色能力(脱色率为92. 69%).Six strains of bacteria with capacity of decolorizing dyes were isolated from activated sludge.Strain Y1,with excellent ability of dye-decolorizing,was identified as Shewanella sp.based on morphological and physiological-biochemical characteristics and 16S rRNA gene sequence analysis.After mixed cultivating,the decolorizing rate of reactive black dye was obviously improved,and the decolorizing rate of most multiple colonies steadied at more than 90%after 72 h.It was found that the decolorizing rate of multiple colony Y1+Y5 was 95.68%,and it has excellent ability of dye-decolorizing for industrial printing and dying wastewater(decolorizing rate is 92.69%).

关 键 词：活性黑染料 脱色菌 分离鉴定 复合菌群 

分 类 号：Q939.97[生物学—微生物学]