人乳头瘤病毒和解脲脲原体、沙眼衣原体、淋病奈瑟菌的感染现状分析  

Infection Status of Human Papilloma Virus,Ureaplasma Urealyticum,Chlamydia Trachomatis,and Neisseria Gonorrhoeae

作　　者：张睿[1] 周艳丽 窦亚玲[1] 孔令君[1] 叶阿里[1] 吴洁[1] 徐英春[1] ZHANG Rui;ZHOU Yanli;DOU Yaling;KONG Lingjun;YE Ali;WU Jie;XU Yingchun(Department of Clinical Laboratory,PUMC Hospital,CAMS and PUMC,Beijing 100730,China;Department of Clinical Laboratory,Yutian County Hospital,Tangshan City,Hebei Province,Yutian,Hebei 064100,China)

机构地区：[1]中国医学科学院北京协和医学院北京协和医院检验科,北京100730 [2]河北省唐山市玉田县医院检验科,河北玉田064100

出　　处：《中国医学科学院学报》2018年第6期817-821,共5页Acta Academiae Medicinae Sinicae

摘　　要：目的分析人乳头瘤病毒(HPV)、解脲脲原体(UU)、沙眼衣原体(CT)、淋病奈瑟菌(NG)在患者中的感染现状。方法收集2014年1月1日至2017年12月31日就诊于北京协和医院门诊患者的870份样本(尿液、尿道拭子和宫颈拭子),采用多重荧光PCR技术检测HPV-DNA;荧光核酸恒温扩增检测技术检测样本中UU-RNA、CT-RNA和NG-RNA,分别计算各种病原体的阳性检出率及不同性别间阳性检出率。按HPV-DNA结果将上述样本分成HPV阳性组和HPV阴性组,对两组中UU-RNA、CT-RNA、NG-RNA的检出率进行统计分析。结果检出率最高是UU-RNA,为53. 68%(467/870),其次是HPV-DNA,总检出率为32. 41%(282/870),最低是NG-RNA,为2. 18%(19/870);高危型(HR)HPV-DNA (型别:16、18、31、33、35、39、45、51、52、56、58、59和68)女性检出率[31. 52%(209/663)]明显高于男性[17. 39%(36/207)],UU-RNA女性总检出率[60. 93%(404/663)]明显高于男性[30. 34%(63/207)],差异有统计学意义(P均<0. 001)。HR-HPV阳性组男性CT检出率[22. 58%(7/31)]明显高于HR-HPV阴性组[4. 54%(8/176)](P <0. 001); HR-HPV阳性组女性CT检出率[10. 5%(21/200)]明显高于HR-HPV阴性组[5. 61%(26/463)](P=0. 024)。女性在低危型(LR)-HPV (型别6、11)阳性组中,UU检出率[70. 83%(34/48)]明显高于LRHPV阴性组[2. 11%(13/615)](P <0. 001)。妇科门诊和皮肤性病门诊检出率最高均为UU-RNA,分别为60. 61%和46. 28%。结论相较于男性,HR-HPV和UU在女性中普遍易感。在HR-HPV阳性患者中,较易联合感染CT。在LR-HPV阳性的女性患者中联合感染UU较为常见。Objective To analyze the infection status of human papilloma virus(HPV),Ureaplasma urealyticum(UU),Chlamydia trachomatis(CT),and Neisseria gonorrhoeae(NG)in clinical patients.Methods The laboratory specimens including urine,urethral swabs,and cervical swabs from 870 patients from January 1st 2014 to December 31st 2017 were retrospectively analyzed.HPV-DNA was detected by multiplex fluorescent PCR,and the UU-RNA,CT-RNA,and NG-RNA were determined by isothermal nucleic acid amplification.The positive rate of each pathogen and the distribution of positive rate between male and female patients were calculated.The samples were further divided into HPV-positive group and HPV-negative group,and the positive rates of UU-RNA,CT-RNA,and NG-RNA in these two groups were compared.Results The highest positive rate was 53.68%(467/870)for UU-RNA,followed by HPV-DNA[32.41%(282/870)]and NG-RNA[2.18%(19/870)].The total positive rate of high-risk(HR)-HPV(subtypes:16,18,31,33,35,39,45,51,52,56,58,59,and 68)[31.52%(209/663)]and UU in female patients[60.93%(404/663)]was significantly higher than that in male patients[17.39%(36/207),30.34%(63/207)](both P<0.001).The male patients had significantly higher CT positive rate in HR-HPV-positive group than in HR-HPV-negative group[22.58%(7/31)vs.4.54%(8/176)](P<0.001).The female patients had significantly higher CT positive rate in HR-HPV-positive group than in HR-HPV-negative group[10.5%(21/200)vs.5.61%(26/463)](P=0.024).The UU-RNA positive rate of females in the low-risk(LR)-HPV(subtypes:6 and 11)positive group was significantly higher than that in LR-HPV negative group[70.83%(34/48)vs.2.11%(13/615)](P<0.001).Conclusions Women are more susceptible to HR-HPV and UU infections.HR-HPV-positive patients are more likely to experience CT infection.In contrast,co-infection with UU is more common in LR-HPV-positive females.

关 键 词：人乳头瘤病毒 宫颈癌 解脲脲原体 沙眼衣原体 淋病奈瑟菌 

分 类 号：R446.9[医药卫生—诊断学]