日光照周期对大菱鲆幼鱼摄食、消化酶活力与血清激素含量的影响  

EFFECTS OF DAILY PHOTOPERIOD ON FEED CONSUMPTION, DIGESTIVE ENZYME ACTIVITIES AND BLOOD HORMONE OF TURBOT SCOPHTHALMUS MAXIMUS L. JUVENILES

作　　者：李宝山 孙永智 王世信 黄炳山 王际英 LI Bao-Shan;SUN Yong-Zhi;WANG Shi-Xin;HUANG Bing-Shan;WANG Ji-Ying(Shandong Marine Resource and Environment Research Institute,Shandong Provincial Key Laboratory of Restoration for Marine Ecology,Yantai 264006,China)

机构地区：[1]山东省海洋资源与环境研究院山东省海洋生态修复重点实验室,烟台264006

出　　处：《水生生物学报》2019年第1期69-77,共9页Acta Hydrobiologica Sinica

基　　金：山东省重点研发计划(2016GSF115005)资助~~

摘　　要：研究了4种光照周期[24L﹕0D(D1)、16L﹕8D(D2)、8L﹕16D(D3)和0L﹕24D(D4)]对体重(30.5±2.0) g大菱鲆Scophthalmus maximus L.幼鱼20h (8:00am—4:00am)内摄食、消化酶活力、血清激素含量的影响。结果显示:(1)实验鱼的摄食率随光照时间的缩短而降低; D1组实验鱼每隔8h出现摄食高峰,其他组均在8:00am及4:00pm出现摄食高峰。(2)D1、D2和D3组在12:00am和8:00pm出现肠道蛋白酶及淀粉酶活力峰值,脂肪酶活力显著高于D4组(P<0.05)。(3)各组8:00am至8:00pm生长激素(GH)含量无显著变化, D1组4:00am时显著高于其他组(P<0.05), D4组0:00am及4:00am显著低于其他组(P<0.05); D1、D2和D3组初次摄食8h内皮质醇(COR)含量无显著变化, 8h后先升高后降低, D4组COR含量先升高后降低, 8:00pm时达到最高; D1和D2组0:00am时去甲肾上腺素(NE)含量显著高于其他组(P<0.05), D4组8:00pm时显著低于其他组(P<0.05); D2组8:00am及12:00am时三碘甲状原氨酸(T3)含量显著高于其他组(P<0.05), D4组8:00pm时显著低于其他组,0:00am时显著高于其他组(P<0.05), D2和D3组4:00am显著低于D1和D4组(P<0.05),各组T3最高值均出现在8:00pm。在实验条件下,光照周期影响了大菱鲆幼鱼摄食、消化酶活力及血清激素含量。在此光照强度下,大菱鲆养殖中以8—16h光照周期、日投喂2次为宜。This study was conducted to investigate the effects of 4 kinds[24L﹕0D(D1),16L﹕8D(D2),8L﹕16D(D3),0L﹕24D(D4)]of photoperiods on feed consumption,digestive enzyme activities and blood hormone of turbot Scophthalmus maximus L.juveniles with initial body weight of(30.5±2.0)g in 20 hours(8:00am-4:00am).The results showed that the feed rate decreased with the shorten of illumination time,and feeding peak in D1 group appeared every 8h,meanwhile others appeared at 8:00am and 4:00pm.Activities of protease and amylase of former 3 groups were appeared spike at 12:00am and 8:00pm,and lipase was significantly higher than that of the D4 group.There were no difference of GH contents from 8:00am to 8:00pm among all groups,and D1 group at 4:00am was significantly higher than others,but D4 group was significantly lower than others from 0:00am to 4:00am.COR contents of D1,D2 and D3 groups were not significant difference in 8h after first feeding,and then increased firstly and decreased afterwards;D4 group reached the maximum level at 8:00pm.NE content of D1 and D2 groups were significantly higher than that of the others at 0:00am,meanwhile D4 group was significantly lower than others at 8:00pm.T3 content of D2 group was significantly higher than that of the other groups at 8:00am and 12:00am;D4 group was lower at 8:00pm,and higher at 0:00am than others;D2 and D3 group were lower than those of D1 and D4 group at 4:00am;The highest values of T3 of all groups were appeared at 8:00pm.These results demonstrated that feed intake,digestive enzyme activities and serum hormone contents of turbot juvenile were affected by photoperiod under current experimental conditions.8-16 lighting period and feed twice daily would be recommended for turbot culture under this intensity of illumination.

关 键 词：大菱鲆 光照周期 摄食 消化酶 激素 

分 类 号：S963.1[农业科学—水产养殖]