丹参提取物中脂溶性成分检测及体外溶出行为考察  

Determination of Lipophilic Components in Extracts of Salvia Miltiorrhiza and Investigation of Its Dissolution Behavior In Vitro

作　　者：高秀容 许小红[1] 杨恒博 张旭[1] 李望[1] 汪少 GAO Xiurong;XU Xiaohong;YANG Hengbo;ZHANG Xu;LI Wang;WANG Shao(School of Pharmacy, Chengdu Medical College, Chengdu 610083, China)

机构地区：[1]成都医学院药学院,成都610083

出　　处：《医药导报》2019年第1期79-83,共5页Herald of Medicine

基　　金：四川省学术技术带头人后备人选培养基金资助项目

摘　　要：目的建立丹参脂溶性成分丹参酮Ⅰ、丹参酮ⅡA及隐丹参酮高效液相色谱(HPLC)同步测定方法,并考察其体外溶出行为。方法选用Agilent HC-C18(4.6 mm×250 mm,5μm)色谱柱,以乙腈-水(含0.1%甲酸)为流动相梯度洗脱,同时测定丹参酮Ⅰ、丹参酮ⅡA及隐丹参酮含量,考察十二烷基硫酸钠(SDS)浓度、溶出液p H值和溶出仪转速对3种成分溶出行为的影响。结果丹参酮Ⅰ回归方程为Y=0. 202 3X+0. 296 7 (R2=0. 999 2),线性范围为1. 45～93.00μg·m L^(-1);丹参酮ⅡA回归方程为Y=0.445 6X+0.527 2(R2=0.999 4),线性范围1.29～82.50μg·m L^(-1);隐丹参酮回归方程为Y=0.131 8X+0.000 2(R2=1.000),线性范围0.90～57.50μg·m L^(-1),方法精密度和回收率均良好,随着溶出液中SDS浓度的增加,3种脂溶性成分溶出度逐渐增加,溶出液p H值及溶出仪转速对3种成分的体外溶出均无显著影响。结论所建立的HPLC方法能准确、灵敏地同时检测丹参3种脂溶性成分,SDS的加入能促进丹参3种脂溶性成分的溶出,溶出液的p H值和溶出仪转速对3种成分的体外溶出无影响。Objective To establish a method of simultaneous determination of tanshinoneⅠ,tanshinonⅡA and cryptotanshinone,three liposoluble constitue of salvia miltiorrhiza and investigate of its dissolution behavior in vitro.Methods An HPLC gradient elution method and Agilent HC-C18(4.6mm×250mm,5μm)column was used in this study the mobile phase was acetonitrile-water(0.1%acetate acid).Contents of the three liposoluble components were determinated simultaneously.And the effects on the three liposoluble components of the SDS concentration,the pH value and the rotation speed of dissolution apparatus were investigated.Results The corresponding linear equation for tanshinoneⅠwas Y=0.2023X+0.2967(R^2=0.9992),the linear range was1.45-93.00μg·mL^-1;the corresponding linear equation for tanshinoneⅡA was Y=0.4456X+0.5272(R^2=0.9994),the linear range was1.29-82.50μg·mL^-1;the corresponding linear equation for cryptotanshinone was Y=0.1318X+0.0002(R^2=1.000),the linear range was0.90-57.50μg·mL^-1,The method had well accuracy and recovery rate,with the increase of SDS concentration in the solution,the dissolution rate three liposoluble components increased gradually.There was no significant effect on the dissolution of the three components in vitro by the pH value of the solution and the rotating speed of the dissolution apparatus.Conclusion The established HPLC methods can detect three liposoluble components of salvia miltiorrhiza accurately and sensitiviely.The addition of SDS can promote the dissolution of three liposoluble components of salvia miltiorrhiza.The pH value of the dissolution solution and the rotational speed of dissolution apparatus had no effects on the dissolution of the three components in vitro.

关 键 词：丹参脂溶性成分 色谱法 高效液相 溶出 体外 

分 类 号：R282.71[医药卫生—中药学] R927.2[医药卫生—中医学]