一个新的黄瓜叶色突变体的光合特性分析  

Photosyntheic Characteristics Analysis of New Leaf Color Mutant in Cucumber

作　　者：胡亮亮[1] 赵子瑶 张海强 陈菲帆 张朝文[1] 戎福喜 陈鹏[2] 李玉红[1] HU Liangliang;ZHAO Ziyao;ZHANG Haiqiang;CHEN Feifan;ZHANG Chaowen;RONG Fuxi;CHEN Peng;LI Yuhong(College of Horticulture,Northwest A&F University,Yangling Shaanxi712100,China;College of Life Science,Northwest A&F University,Yangling Shaanxi712100,China)

机构地区：[1]西北农林科技大学园艺学院,陕西杨凌712100 [2]西北农林科技大学生命科学学院,陕西杨凌712100

出　　处：《西北农业学报》2018年第11期1622-1628,共7页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家自然科学基金(31471891;31772300);西北农林科技大学2015年度国际科技合作项目~~

摘　　要：叶色突变体是研究植物光合系统结构与功能、叶绿素生物合成与代谢以及叶绿体发育的理想材料。该研究以黄瓜黄绿叶突变体C777及其野生型CCMC为试材,对其苗期叶片的光合色素质量分数、光合特性、叶绿素荧光参数等进行测定。结果表明,突变体叶绿素a、叶绿素b、类胡萝卜素以及总叶绿素质量分数均显著低于野生型;光合特性方面,突变体净光合速率显著低于野生型,而胞间CO_2浓度显著高于野生型,表明突变体对CO2的利用率较野生型低;叶绿素荧光动力学参数的测定结果表明,突变体的F_o、F_m、F_v/F_m、F′_v/F′_m、Φ_(PSⅡ)、qP、ETR和NPQ均显著降低,说明光合色素质量分数的降低导致PSⅡ反应中心捕光能力较弱和光化学转化效率较低,使得突变体光合速率降低。试验为进一步阐明该黄瓜黄绿叶突变体的叶色突变机制和该种质的合理利用提供相关基础资料。Leaf color mutant is an ideal material for studying photosynthesis system structure and function,chlorophyll biosynthesis and metabolism,and chloroplast development.The mutant C777and wild type CCMC were taken as materials.The photosynthetic pigment mass fraction,photosynthetic characteristics,chlorophyll fluorescence parameters were measured in this study.The results showed that chlorophyll a,chlorophyll b,carotenoid and total chlorophyll mass fraction of mutant were significantly lower than those of wild type.In photosynthetic characteristics,the net photosynthetic rate of the mutant was significantly lower than that of the wild type,while the intercellular CO2concentration was significantly higher than that of the wild type,it indicated that the utilization of CO2by mutant was lower than wild type.The determination of chlorophyll fluorescence kinetic revealed that the mutant significantly decreased in F o,F m,F v/F m,F′v/F′m,ΦPSⅡ,q P,ETR and NPQ,this suggested that the reduction in photosynthetic pigment mass fraction led to low PSⅡreaction,light harvest ability,and less photochemical conversion efficiency,so the mutant photosynthetic rate decreased.These results provide basic information for further elucidating mechanism of the leaf color mutation and rational utilization of the germplasm in the virescent leaf mutant in cucumber.

关 键 词：黄瓜 黄绿叶突变体 光合特性 色素质量分数 叶绿素荧光 

分 类 号：S642.2[农业科学—蔬菜学]